Blm Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86827 McDaniel LD, et al., Chromosome instability and tumor predisposition inversely correlate with BLM protein levels. DNA Repair (Amst). 2003 Dec 9;2(12):1387-404
Assay type:	Western blot
MGI Accession ID:	MGI:3511299
Gene symbol:	Blm
Gene name:	Bloom syndrome, RecQ like helicase

Antibody:	anti-Blm
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The authors state that animals of the genotypes Blmtm3Brd/Blm+, Blmtm1Ches/Blm+, Blmtm3Brd/Blmtm3Brd, and Blmtm1Ches/Blmtm3Brd produced protein at levels of 62, 50, 25 and 12% of that seen in Blm+/Blm+ controls, respectively.

Results

Image: 5C

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	87 kDa (a)	53 kDa (b)	Size Not Specified (c)	Genetic Background	Mutant Allele(s)	Sex	Note
+/+, 1	E13.5	TS21: liver	Absent	Absent	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss		Not Specified
+/+, 2	E13.5	TS21: liver	Absent	Absent	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss		Not Specified	2-fold dilution
+/+, 4	E13.5	TS21: liver	Absent	Absent	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss		Not Specified	4-fold dilution
+/+, 8	E13.5	TS21: liver	Absent	Absent	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss		Not Specified	8-fold dilution
-/-, 1	E13.5	TS21: liver	Present	Present	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss	Blm^tm1Ches/Blm^tm3Brd	Not Specified
-/-, 1	E13.5	TS21: liver	Present	Present	Present	involves: 129S/SvEv * C57BL/6 * NIH Black Swiss	Blm^tm1Ches/Blm^tm3Brd	Not Specified
-/-	E13.5	TS21: liver	Absent	Absent	Absent	involves: 129S6/SvEvTac * NIH Black Swiss	Blm^tm3Ches/Blm^tm3Ches	Not Specified
+/+	E13.5	TS21: liver	Absent	Absent	Present	involves: 129S6/SvEvTac * NIH Black Swiss		Not Specified

Notes:
(a) The authors predict that this is a truncated protein from the tm1Ches1 allele.
(b) The authors predict that this is a truncated protein from the tm3Brd allele.
(c) The authors state that this represents the full-length protein.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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