Blm Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86827 McDaniel LD, et al., Chromosome instability and tumor predisposition inversely correlate with BLM protein levels. DNA Repair (Amst). 2003 Dec 9;2(12):1387-404
Assay type:	Western blot
MGI Accession ID:	MGI:3511297
Gene symbol:	Blm
Gene name:	Bloom syndrome, RecQ like helicase

Antibody:	anti-Blm
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 5B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	53 kDa (a)	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex	Note
8, +/+	E13.5	TS21: liver	Absent	Present	involves: 129S7/SvEvBrd		Not Specified	12.5% diluted liver extract.
4, +/+	E13.5	TS21: liver	Absent	Present	involves: 129S7/SvEvBrd		Not Specified	25% diluted liver extract.
2, +/+	E13.5	TS21: liver	Absent	Present	involves: 129S7/SvEvBrd		Not Specified	50% diluted liver extract.
1, +/+	E13.5	TS21: liver	Absent	Present	involves: 129S7/SvEvBrd		Not Specified	Undiluted liver extract.
1, -/-	E13.5	TS21: liver	Present	Present (c)	involves: 129S7/SvEvBrd	Blm^tm3Brd/Blm^tm3Brd	Not Specified	Undiluted liver extract.

Notes:
(a) The authors predict that this is the truncated protein.
(b) The authors state that this represents the full-length protein.
(c) The authors state that the mutant produces 25% of normal levels of the full-length protein.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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