Blm RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:86827 McDaniel LD, et al., Chromosome instability and tumor predisposition inversely correlate with BLM protein levels. DNA Repair (Amst). 2003 Dec 9;2(12):1387-404
Assay type:	RT-PCR
MGI Accession ID:	MGI:3511292
Gene symbol:	Blm
Gene name:	Bloom syndrome, RecQ like helicase

Probe:	Blm-pA, Blm-pB
Probe preparation:	DNA
Visualized with:	Ethidium bromide
Assay notes:	This RT-PCR reaction was performed using primers designed to detect a tandem duplication of exon 4 mRNA sequence.

Results

Image: 4D upper

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	Control
-/-	P adult	TS28: brain	Present	Not Specified	involves: 129S7/SvEvBrd	Blm^tm3Brd/Blm^tm3Brd	Not Specified
+/+	Control
-/-	P adult	TS28: testis	Present	Not Specified	involves: 129S7/SvEvBrd	Blm^tm3Brd/Blm^tm3Brd	Male

Notes:
(a) The primers used in this assay are designed to amplify the mutated product only.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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