Cyp17a1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:21125 Lee CH, et al., Normal onset, but prolonged expression, of Sry gene in the B6.YDOM sex-reversed mouse gonad. Dev Biol. 1994 Oct;165(2):442-52
Assay type:	RT-PCR
MGI Accession ID:	MGI:3497946
Gene symbol:	Cyp17a1
Gene name:	cytochrome P450, family 17, subfamily a, polypeptide 1

Probe:	17-HA-pA,-pB
Probe preparation:	DNA
Visualized with:	Ethidium bromide
Assay notes:	Analyses were performed in C57BL/6J mice which contained the Y chromosome of M. m. domesticus. A large number of gonads were examined and a summary of RT-PCR analyses in B6.YDom is shown as a percentage of positive results in Figure 4.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	396 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
S	Mol. Wt. Marker Lane
10,+	E10.5 (a)	TS17: gonad primordium	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Morphologically undifferentiated gonad.
10,-	Control
11,+	E11.5 (a)	TS19: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Morphologically undifferentiated gonad.
11,-	Control
12,+	E12.5 (a)	TS20: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Morphologically undifferentiated gonad.
12,-	Control
S	Mol. Wt. Marker Lane
13,+	E13.5 (a)	TS21: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovary (sex-reversed).
13,-	Control
14,+	E14.5 (a)	TS22: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovary (sex-reversed).
14,-	Control
15,+	E15.5 (a)	TS23: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovary (sex-reversed).
15,-	Control
16,+	E16.5 (a)	TS24: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovary (sex-reversed).
16,-	Control
18,+	E18.5 (a)	TS26: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovary (sex-reversed).
18,-	Control
S	Mol. Wt. Marker Lane
13,+	E13.5 (a)	TS21: gonad	Absent	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovotestis.
13,-	Control
14,+	E14.5 (a)	TS22: gonad	Present	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovotestis.
14,-	Control
15,+	E15.5 (a)	TS23: gonad	Weak	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovotestis.
15,-	Control
16,+	E16.5 (a)	TS24: gonad	Weak	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovotestis.
16,-	Control
18,+	E18.5 (a)	TS26: gonad	Weak	Not Specified; total RNA	involves: C57BL/6J * M. m. domesticus		Not Specified	Ovotestis.
18,-	Control

Notes:
(a) Age normalized so that noon of plug day = E0.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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