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Gene Expression Data
Assay Details
Assay
Reference: J:21125 Lee CH, et al., Normal onset, but prolonged expression, of Sry gene in the B6.YDOM sex-reversed mouse gonad. Dev Biol. 1994 Oct;165(2):442-52
Assay type: RT-PCR
MGI Accession ID: MGI:3497911
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Probe: Sry-pI, Sry-pJ
Probe preparation: DNA
Visualized with: Ethidium bromide
Assay notes: A large number of gonads were examined and a summary of RT-PCR analyses is shown as a percentage of positive results in Figure 2.
Results
Image: 1a ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 349 bp Amount Genetic BackgroundMutant Allele(s)Sex
S Mol. Wt. Marker Lane
10,+ E10.5 (a) TS17: gonad primordium Present (b) Not Specified; total RNA C57BL/6J Male
10,- Control
11,+ E11.5 (a) TS19: gonad Strong Not Specified; total RNA C57BL/6J Male
11,- Control
12,+ E12.5 (a) TS20: testis Present Not Specified; total RNA C57BL/6J Male
12,- Control
13,+ E13.5 (a) TS21: testis Present (c) Not Specified; total RNA C57BL/6J Male
13,- Control
14,+ E14.5 (a) TS22: testis Absent Not Specified; total RNA C57BL/6J Male
14,- Control
15,+ E15.5 (a) TS23: testis Absent Not Specified; total RNA C57BL/6J Male
15,- Control
16,+ E16.5 (a) TS24: testis Absent Not Specified; total RNA C57BL/6J Male
16,- Control
18,+ E18.5 (a) TS26: testis Absent Not Specified; total RNA C57BL/6J Male
18,- Control
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The authors state that a minority of gonads were positive by RT-PCR.
(c) The authors state that only one gonad was positive by RT-PCR.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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