Tg(Ins1-Frk-Y504F)1Ubc
Transgene Detail
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| Symbol: |
Tg(Ins1-Frk-Y504F)1Ubc |
| Name: |
transgene insertion 1, Uppsala University |
| MGI ID: |
MGI:3487260 |
| Synonyms: |
GTK-Y504F, GTKY504F, RIP-GTK |
| Transgene: |
Tg(Ins1-Frk-Y504F)1Ubc Location: unknown
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| Alliance: |
Tg(Ins1-Frk-Y504F)1Ubc page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Ins1-Frk-Y504F)1Ubc expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Frk (MGI:103265) |
Transgene codes for a protein with a tyrosine to phenylalanine mutation. |
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Mutation details: The transgene comprises a 0.41-kb DNA fragment containing the rat insulin 1 promoter, a 1.6-kb mouse cDNA encoding an in-vitro mutated variant of fyn-related kinase (FYN) joined to a cDNA fragment encoding a MYC epitope tag, and the polyadenylation signal of the bovine growth hormone gene. The mutant fyn-related kinase protein has a substitution of phenylalanine for tyrosine at amino acid position 504, which confers enhanced kinase activity. Expression of transgene-derived mRNA was demonstrated by polymerase chain reaction of islet cDNA using a primer matching the Myc sequence. Only moderate elevation of FYN protein was detected in islet lysates or in FYN immunoprecipitates using unpurified anti-FYN antiserum.
(J:93623)
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| Inheritance: |
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Not Specified |
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:93623 Anneren C, et al., Increased cytokine-induced cytotoxicity of pancreatic islet cells from transgenic mice expressing the Src-like tyrosine kinase GTK. Mol Med. 2001 May;7(5):301-10 |
| All: |
3 reference(s) |
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Analysis Tools
