Akr1b7 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:85720 Baron S, et al., Hormonal and developmental regulation of the mouse aldose reductase-like gene akr1b7 expression in Leydig cells. J Mol Endocrinol. 2003 Aug;31(1):71-81
Assay type:	Northern blot
MGI Accession ID:	MGI:3055542
Gene symbol:	Akr1b7
Gene name:	aldo-keto reductase family 1, member B7

Probe:	akr1b7 cDNA
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	A graph of expression levels is presented in Fig. 4C.

Results

Image: 4A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E16.5	E17.0 (a)	TS25: testis	Weak	30 µg; total RNA	CD-1		Male
1	P1	TS27: testis	Present	30 µg; total RNA	CD-1		Male
3	P3	TS27: testis	Present	30 µg; total RNA	CD-1		Male
6	P6	TS28: testis	Present (b)	30 µg; total RNA	CD-1		Male
10	P10	TS28: testis	Present	30 µg; total RNA	CD-1		Male
16	P16	TS28: testis	Present (c)	30 µg; total RNA	CD-1		Male
20	P20	TS28: testis	Present	30 µg; total RNA	CD-1		Male
24	P24	TS28: testis	Present	30 µg; total RNA	CD-1		Male
30	P30	TS28: testis	Present	30 µg; total RNA	CD-1		Male
300	P300	TS28: testis	Present	30 µg; total RNA	CD-1		Male

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) This is the time of peak expression.
(c) Expression returns to basal levels at this point, in contrast to the protein levels which remain elevated (MGI:3055544).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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