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Gene Expression Data
Assay Details
Assay
Reference: J:85657 Greene RM, et al., Intracellular dynamics of Smad-mediated TGFbeta signaling. J Cell Physiol. 2003 Nov;197(2):261-71
Assay type: Northern blot
MGI Accession ID: MGI:3055454
Gene symbol: Smad4
Gene name: SMAD family member 4
Probe: Smad4 cDNA2
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: This Northern blot is representative of at least three separate Northern blots of unique sets of RNA extracted from palate.
Results Image: 2_Smad4
 Sample Information Bands Other Sample Information
Lane AgeStructure 4 kb (b) Amount Genetic BackgroundMutant Allele(s)Sex
GD 12 E12.5 (a) TS20: palatal shelf
TS20: primary palate
Present 20 µg; total RNA ICR Not Specified
GD 13 E13.5 (a) TS21: palatal shelf
TS21: primary palate
Present 20 µg; total RNA ICR Not Specified
GD 14 E14.5 (a) TS22: palatal shelf
TS22: primary palate
Present 20 µg; total RNA ICR Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels decreased during the time examined.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/30/2024
MGI 6.23
The Jackson Laboratory