Srf Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91025 Parlakian A, et al., Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality. Mol Cell Biol. 2004 Jun;24(12):5281-9
Assay type:	Western blot
MGI Accession ID:	MGI:3054949
Gene symbol:	Srf
Gene name:	serum response factor

Antibody:	anti-Srf
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 1F

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	67 kDa	54 kDa	46 kDa (b)	Genetic Background	Mutant Allele(s)	Sex	Note
- wt	E10.5 (a)	TS17: heart	Present	Present	Absent	Not Specified		Not Specified
- S^f/S^f	E10.5 (a)	TS17: heart	Present (c)	Present (c)	Absent	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli	Not Specified
- S^f/S^f	E10.5 (a)	TS17: heart	Absent	Absent	Present	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli, Tg(Myh7-cre)1Zli/0	Not Specified	Conditional mutant.
- S^f/S^f	E10.5 (a)	TS17: heart	Absent	Absent	Present	involves: 129/Sv * C57BL/6	Srf^tm1Zli/Srf^tm1Zli	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band represents the truncated protein.
(c) There is no difference in expression between mutants and wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24