Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57761
Singer JD, et al., Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 1999 Sep 15;13(18):2375-87
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3054623
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Gene symbol:
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Ccne1
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Gene name:
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cyclin E1
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Probe:
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Cyclin E cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Results of this assay are not shown.
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Results
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Specimen
E7.5, +/+:
embryonic day 7.5
(more  )
Specimen
E7.5, +/+:
(close  )
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Genetic Background:
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involves: 129S4/SvJaeSor * C57BL/6J
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
E7.5, -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor * C57BL/6J
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Mutant Allele(s):
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Cul3tm1Jro/Cul3tm1Jro
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
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The authors state that the in situ analyses did not reveal an increased expression of mRNA in mutant embryos (as opposed to increased protein levels, as seen in assay MGI:3054571).
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| TS11: extraembryonic component |
Present |
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The authors state that the in situ analyses did not reveal an increased expression of mRNA in mutant embryos (as opposed to increased protein levels, as seen in assay MGI:3054571).
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Analysis Tools
