Tg(tetop-lacZ)2Mam Transgene Detail MGI Mouse (MGI:3053973)

Tg(tetop-lacZ)2Mam
Transgene Detail

Summary

Symbol:	Tg(tetop-lacZ)2Mam
Name:	transgene insertion 2, Lothar Hennighausen
MGI ID:	MGI:3053973
Synonyms:	TetO-beta-gal, tTAluc
Transgene:	Tg(tetop-lacZ)2Mam Location: unknown
Alliance:	Tg(tetop-lacZ)2Mam page

Transgene
origin

Strain of Origin:

C57BL/6 x SJL

Transgene
description

Transgene Type:		Transgenic (Inducible, Reporter)
Inducer:		doxycycline/tetracycline
Mutation:		Insertion
		Mutation details: The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. (J:92587)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Four lines were produced (G1-G4).

In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene.

References

Original:	J:92587 Furth PA, et al., Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9302-6
All:	16 reference(s)

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