Arhgap35 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:90071 Hernandez SE, et al., Adhesion-dependent regulation of p190RhoGAP in the developing brain by the Abl-related gene tyrosine kinase. Curr Biol. 2004 Apr 20;14(8):691-6
Assay type:	Western blot
MGI Accession ID:	MGI:3053829
Gene symbol:	Arhgap35
Gene name:	Rho GTPase activating protein 35

Antibody:	anti-PY
Assay notes:	Lanes contain insoluble fractions of brain extracts.

Results

Image: 1A PY

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	190 kDa	Genetic Background	Mutant Allele(s)	Sex
E16 WT	E16.0 (a)	TS24: brain	Present (b)	Not Specified		Not Specified
E16 arg-/-	E16.0 (a)	TS24: brain	Present (c)	involves: 129S4/SvJae * C57BL/6J	Abl2^tm1Ajk/Abl2^tm1Ajk	Not Specified
P11 WT	P11	TS28: brain	Strong (d)	Not Specified		Not Specified
P11 arg-/-	P11	TS28: brain	Present (e)	involves: 129S4/SvJae * C57BL/6J	Abl2^tm1Ajk/Abl2^tm1Ajk	Not Specified
P21 WT	P21	TS28: brain	Strong (d)	Not Specified		Not Specified
P21 arg-/-	P21	TS28: brain	Present (f)	involves: 129S4/SvJae * C57BL/6J	Abl2^tm1Ajk/Abl2^tm1Ajk	Not Specified
Adult WT	P w 8	TS28: brain	Present (b)	Not Specified		Not Specified
Adult arg-/-	P w 8	TS28: brain	Present (c)	involves: 129S4/SvJae * C57BL/6J	Abl2^tm1Ajk/Abl2^tm1Ajk	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report levels are lower at E16 and 8-weeks compared with P11 and P21
(c) Little difference between arg-/- and wild-type at these time points.
(d) Signal is strongest in wild-type mice at P11 and P21.
(e) Signal is decreased 3-fold compared with wild-type.
(f) Signal is decreased 2-fold compared with wild-type.

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