Tg(MTn-lacZ)204Bri Transgene Detail MGI Mouse (MGI:3052912)

Tg(MTn-lacZ)204Bri
Transgene Detail

Summary

Symbol:	Tg(MTn-lacZ)204Bri
Name:	transgene insertion 204, Ralph L Brinster
MGI ID:	MGI:3052912
Transgene:	Tg(MTn-lacZ)204Bri Location: unknown
Alliance:	Tg(MTn-lacZ)204Bri page

Transgene
origin

Strain of Origin:

C57BL/6J

Transgene
description

Transgene Type:		Transgenic (Reporter)
Mutation:		Insertion
		Mutation details: The mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression. (J:92451)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions.

References

Original:	J:92451 Rhim JA, et al., Replacement of diseased mouse liver by hepatic cell transplantation. Science. 1994 Feb 25;263(5150):1149-52
All:	3 reference(s)

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