Antibody:	[a]1CT-FP
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Very strong	Regionally restricted	1A	Staining is in the neuroepithelium.
TS13: migrating neural crest	Very strong		1A
TS13: surface ectoderm	Very strong	Single cells	1A	Expression is in scattered cells.

Structure	Level	Pattern	Image	Note
TS13: head somite	Very strong
TS13: trunk somite	Very strong
TS13: embryo	Very strong	Regionally restricted		Expression is in the mesoderm and in scattered cells in the endoderm.
TS13: head mesenchyme	Very strong	Regionally restricted		Expression is in the cephalic mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1B	Expression is in the basal surface of the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present	Regionally restricted	1C	Expression is also in the neuronal projections.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present	Regionally restricted	1D	Expression is in the ventral neural tube in the axonal projections that enter and exit the structure.
TS19: dorsal root ganglion	Strong	Regionally restricted	1D	Expression is in a subset of cells.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		1E

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong	Regionally restricted	1F	Expression is in a subset of cells.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Strong		1G
TS19: dorsal root ganglion	Strong	Regionally restricted	1G	Expression is in a subset of cells.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata part of 4th ventricle choroid plexus	Present		1H
TS20: metencephalon part of 4th ventricle choroid plexus	Present		1H

Structure	Level	Pattern	Image	Note
TS23: vagus ganglion	Present		1J

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Strong	Regionally restricted	1K	Expression is in the neuronal projection.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Trace	Regionally restricted		The expression is reduced to background levels in the white matter only, expression is in the ependymal cells.
TS23: brain	Present	Regionally restricted		Expression is in the ependymal cells.
TS23: choroid plexus	Present
TS23: seminiferous cord	Present
TS23: collecting duct	Present
TS23: clavicle	Absent
TS23: cranium	Absent
TS23: esophagus	Absent
TS23: liver	Absent
TS23: maturing glomerular tuft	Absent
TS23: limb digit	Absent			Expression is absent in the bones.
TS23: heart	Weak
TS23: skeletal muscle	Present

Structure	Level	Pattern	Image	Note
TS17: infundibular recess of 3rd ventricle	Strong	Graded	1L	Expression is especially concentrated in the basolateral regions.
TS17: Rathke's pouch	Absent		1L

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present	Regionally restricted	1M	Expression is in a subpopulation of cells.
TS15: infundibular recess of 3rd ventricle	Present	Regionally restricted	1M	Expression is in the basal surface.

Structure	Level	Pattern	Image	Note
TS27: capillary	Present	Regionally restricted	2A	Expression is in brain capillaries.

Structure	Level	Pattern	Image	Note
TS28: capillary	Present	Regionally restricted	2B	Expression is in the brain capillaries.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		3A
TS15: optic vesicle	Absent		3A

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		3B
TS15: optic vesicle	Absent		3B

Structure	Level	Pattern	Image	Note
TS17: optic cup outer layer	Present		3C
TS17: lens pit	Weak		3C

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		3D
TS15: optic vesicle	Absent		3D

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Strong		3E
TS19: pigmented retinal epithelium	Present		3E
TS19: lens vesicle	Weak		3E
TS19: cornea epithelium	Absent		3E
TS19: perioptic mesenchyme	Absent		3E

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Strong		3F
TS19: neural retinal epithelium	Weak		3F
TS19: cornea epithelium	Absent		3F
TS19: perioptic mesenchyme	Absent		3F

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Single cells	4A
TS15: surface ectoderm	Present	Single cells	4A

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Strong	Regionally restricted	4B	Expression is at the basolateral region in contact with the facio-acoustic (VII-VIII) neural crest complex.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present	Regionally restricted	4C	Expression is basolateral in the epithelia.
TS20: semicircular canal	Present	Regionally restricted	4C	Expression is basolateral in the epithelia.

Structure	Level	Pattern	Image	Note
TS23: inner ear vestibular component	Strong	Regionally restricted	4D	Expression is in the vestibular hair cells.
TS23: inner ear	Weak	Regionally restricted	4D	Expression is in the basal regions of sensory epithelial cells.

Structure	Level	Pattern	Image	Note
TS23: inner ear vestibular component	Strong	Regionally restricted	4E	Expression is in the vestibular hair cells.
TS23: inner ear	Weak	Regionally restricted	4E	Expression is in the basal regions of sensory epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Strong		5A
TS21: rib pre-cartilage condensation	Strong		5A
TS21: skeletal muscle	Strong		5A

Structure	Level	Pattern	Image	Note
TS23: nasal septum	Absent		5B
TS23: nose	Present	Regionally restricted	5B	Expression is in the nasal glands.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		5C
TS21: outflow tract	Present		5C

Structure	Level	Pattern	Image	Note
TS19: esophagus epithelium	Present		5D	Staining is in the basolateral regions of the epithelial cells.
TS19: trachea epithelium	Present		5D	Staining is in the basolateral regions of the epithelial cells.

Structure	Level	Pattern	Image	Note
TS15: tracheal diverticulum epithelium	Present		5E

Structure	Level	Pattern	Image	Note
TS21: diaphragm	Present		5F
TS21: lung epithelium	Present		5F

Structure	Level	Pattern	Image	Note
TS21: tongue	Present		5G

Structure	Level	Pattern	Image	Note
TS15: gut	Strong		6A

Structure	Level	Pattern	Image	Note
TS17: gallbladder primordium	Present		6B
TS17: stomach epithelium	Present		6B
TS17: foregut	Present	Regionally restricted	6B	Expression is in the duodenum.
TS17: midgut	Present	Regionally restricted	6B	Expression is in the duodenum.

Structure	Level	Pattern	Image	Note
TS20: midgut	Present		6C
TS20: stomach epithelium	Present		6C

Structure	Level	Pattern	Image	Note
TS23: midgut	Present	Regionally restricted	6D	Expression is in the smooth muscle layers and is absent in the epithelium.

Structure	Level	Pattern	Image	Note
TS19: thyroid gland	Present	Spotted	6E inset , 6E	Expression is in a subset of cells.

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Regionally restricted	6F	Expression is in the basal cell layer of the skin of the leg.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	6G	Expression is in the metanephric tubules.
TS20: endocrine gland	Present	Regionally restricted	6G	Expression is in the adrenal primordium.

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Absent		6H
TS15: nephrogenic cord	Absent		6H

Structure	Level	Pattern	Image	Note
TS20: urogenital mesentery	Present	Regionally restricted	6J	Expression is in the caudal part.
TS20: mesonephros	Present	Regionally restricted	6J	Expression is in the ducts and tubules.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	6K	Homogeneous expression is in the metanephric tubules.
TS20: endocrine gland	Present	Regionally restricted	6K	Expression is in the adrenal primordium.

Structure	Level	Pattern	Image	Note
TS20: ureter	Present	Regionally restricted	6L	Expression is in the ureteric bud at the entrance of the urogenital sinus.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	6M	Expression is in selected tubules.
TS21: primary sex cord	Very strong	Patchy	6M

Structure	Level	Pattern	Image	Note
TS27: brain	Absent

Structure	Level	Pattern	Image	Note
TS17: lung	Present	Regionally restricted		Expression is in the main bronchi.
TS17: liver	Absent

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Weak
TS19: heart ventricle	Weak
TS19: liver	Absent
TS19: midgut	Weak	Regionally restricted		Expression is in the epithelia.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted		Expression is in the ependymal cells.
TS20: spinal cord	Present	Regionally restricted		Expression is in the ependymal cells.
TS20: heart	Weak
TS20: skeletal muscle	Strong
TS20: liver	Absent

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted		Expression is in the ependymal cells.
TS21: spinal cord	Present	Regionally restricted		Expression is in the ependymal cells.
TS21: choroid plexus	Present
TS21: clavicle pre-cartilage condensation	Present
TS21: cranium	Present
TS21: limb digit	Present	Regionally restricted		Expression is in the bones.
TS21: midgut	Present	Regionally restricted		Expression in the msucle layers and strong in the epithelium.
TS21: heart	Weak
TS21: skeletal muscle	Present
TS21: primitive collecting duct	Present
TS21: renal cortex	Absent			Absent in the glomeruli.
TS21: liver	Absent

Structure	Level	Pattern	Image	Note
TS28: epidermis	Absent