Cacna1d RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86180 Xu M, et al., Enhanced expression of L-type Cav1.3 calcium channels in murine embryonic hearts from Cav1.2-deficient mice. J Biol Chem. 2003 Oct 17;278(42):40837-41
Assay type:	RT-PCR
MGI Accession ID:	MGI:3050097
Gene symbol:	Cacna1d
Gene name:	calcium channel, voltage-dependent, L type, alpha 1D subunit

Probe:	Dutr01, RACE12
Visualized with:	Ethidium bromide
Assay notes:	The authors have created more than one mutant allele; it was unclear which was used in this assay.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	590 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E9.5 (a)	TS15: heart	Present	4 µg; total RNA	Not Specified		Not Specified
-/-	E9.5 (a)	TS15: heart	Present (b)	4 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Cacna1c^tm1Hfm/Cacna1c^tm1Hfm	Not Specified
+/+	E12.5 (a)	TS20: heart	Present	4 µg; total RNA	Not Specified		Not Specified
-/-	E12.5 (a)	TS20: heart	Present (c)	4 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Cacna1c^tm1Hfm/Cacna1c^tm1Hfm	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression is slightly upregulated in the mutant compared to the wild type.
(c) Expression is upregulated in the mutant compared to the wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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