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Gene Expression Data
Assay Details
Assay
Reference: J:29606 Yoshida M, et al., A new strategy of gene trapping in ES cells using 3'RACE. Transgenic Res. 1995 Jul;4(4):277-87
Assay type: RT-PCR
MGI Accession ID: MGI:3047810
Gene symbol: Rab3ip
Gene name: RAB3A interacting protein
Probe: AS2, S1
Probe preparation: Double stranded DNA
Assay notes: The RT-PCR products were amplified, transferred to a nitrocellulose filter, and then hybridized with a gene specific probe. For their reverse transcription reaction the authors used 1 microgram of total RNA from kidneys and 0.1 microgram of polyA(+) RNAfrom testes. The numbers at the top of the figure indicate different dilutions of the RT-PCR products.
Results Image: 8d lower
 Sample Information Bands Other Sample Information
Lane AgeStructure 380 bp Amount Genetic BackgroundMutant Allele(s)Sex
-/- P adult TS28: metanephros
TS28: testis 		Absent Not Specified involves: C57BL/6 * CBA Rab3ipGt(pPAT)1Sia/Rab3ipGt(pPAT)1SiaMale
+/+ P adult TS28: metanephros
TS28: testis 		Present Not Specified Not Specified Male
+/- P adult TS28: metanephros
TS28: testis 		Present (a) Not Specified involves: C57BL/6 * CBA Rab3ipGt(pPAT)1Sia/Rab3ip+Male
Notes:
(a) Expression levels in the heterozygotes are half those seen in the wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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