Cpt2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:91447 Gentile L, et al., Single-cell quantitative RT-PCR analysis of Cpt1b and Cpt2 gene expression in mouse antral oocytes and in preimplantation embryos. Cytogenet Genome Res. 2004;105(2-4):215-21
Assay type:	RT-PCR
MGI Accession ID:	MGI:3047549
Gene symbol:	Cpt2
Gene name:	carnitine palmitoyltransferase 2

Probe:	Cpt2-pC, Cpt2-pD
Assay notes:	Nested primer sets were used for this analysis. The outer primer set was MGI:3047545. The results illustrated in the paper were obtained using embryos fertilized in vitro, but the authors discuss results obtained with in vivo embryos in the text.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	254 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
MII	P (a)	TS28: oocyte	Present	Not Specified	involves: C3H * C57BL/6		Female
Zyg	E0.5 (a)	TS1: 1-cell stage conceptus	Strong	Not Specified	involves: C3H * C57BL/6		Not Specified
2-C	E1.0 (a)	TS2: 2-cell stage conceptus	Present	Not Specified	involves: C3H * C57BL/6		Not Specified
4-C	E2.0 (a)	TS3: 4-cell stage conceptus	Trace	Not Specified	involves: C3H * C57BL/6		Not Specified
8-C	E2.5 (a)	TS3: 8-cell stage conceptus	Trace	Not Specified	involves: C3H * C57BL/6		Not Specified
Mor	E3.0 (a)	TS3: morula-stage conceptus	Present	Not Specified	involves: C3H * C57BL/6		Not Specified
Bl	E3.5 (a)	TS4: blastocyst-stage conceptus	Present (c)	Not Specified	involves: C3H * C57BL/6		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Results obtained using single embryos suggest that the number of transcripts in each embryo at each time point varies.
(c) This is the period of peak expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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