Probe:	pCRII-TOPO-Bono1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present		2B , 2A
TS24: mandible	Present		2B , 2A
TS24: maxilla	Present		2B , 2A
TS24: nasal septum mesenchyme	Present	Regionally restricted	2B , 2A	Expression is in the ossification regions, weak in cartilage.
TS24: head	Present	Regionally restricted	2B , 2A	Expression is in most ossification regions within the bones, absent in cartilage at the cranial base.
TS24: Meckel's cartilage	Absent		2A , 2B

Structure	Level	Pattern	Image	Note
TS25: mandible	Present		2D , 2C
TS25: maxilla	Present		2D , 2C
TS25: nasal capsule	Present		2D , 2C
TS25: frontal bone	Present		2C , 2D	A higher magnification of frontal suture shows strong expression in osteoblasts up to the osteogenic front.
TS25: head	Present	Regionally restricted	2D , 2C	Expression is in most ossification regions withing the bones, absent in cartilage at the cranial base
TS25: Meckel's cartilage	Absent		2D , 2C

Structure	Level	Pattern	Image	Note
TS23: ulna	Present	Regionally restricted	2E	Expression is in osteoblasts within the distal area of the ossification center of the mid-shaft region.

Structure	Level	Pattern	Image	Note
TS23: radius	Present	Regionally restricted	2F	Expression is within the distal and proximal areas of the ossification center of the mid-shaft region of the radius.

Structure	Level	Pattern	Image	Note
TS23: hand	Absent			Absent in cartilage.
TS23: rib	Absent			Absent in cartilage.
TS23: vertebral cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS16: embryo	Absent

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Absent		3A

Structure	Level	Pattern	Image	Note
TS21: mandible mesenchyme	Present	Regionally restricted	3C	Expression is in maturing osteoblasts in the central portion of the mandibular bone.
TS21: lower jaw tooth	Absent		3C

Structure	Level	Pattern	Image	Note
TS22: mandible	Present		3E
TS22: lower jaw tooth	Absent		3E

Structure	Level	Pattern	Image	Note
TS23: mandible	Present	Graded	3G	Expression is most intense at the periphery of the bone where bone is deposited during periosteal growth.
TS23: lower jaw tooth	Absent		3G	Expression is absent in the dental follicle and the cervical dental papilla mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: mandible	Present	Graded	3I	Expression of is most intense at the periphery of the bone where bone is deposited during periosteal growth.
TS24: lower jaw tooth	Absent		3I	Expression is absent in the dental follicle and the cervical dental papilla mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: mandible	Present	Graded	3K	Expression is most intense at the periphery of the bone where bone is deposited during periosteal growth.
TS25: lower jaw tooth	Absent		3K	Expression is absent in the dental follicle and the cervical dental papilla mesenchyme.

Structure	Level	Pattern	Image	Note
TS27: jaw	Weak	Regionally restricted	4A	Expression is in the mandible.
TS27: tooth	Strong	Regionally restricted	4A	Expression is in the dentine-secreting odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: jaw	Weak	Regionally restricted	4D	Expression is in the mandible.
TS28: tooth	Strong	Regionally restricted	4D	Expression is in the dentine-secreting odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: jaw	Weak	Regionally restricted	4G	Expression is in the mandible.
TS28: tooth	Strong	Regionally restricted	4G	Expression is in the dentine-secreting odontoblasts.