Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75934
Zelzer E, et al., Skeletal defects in VEGF(120/120) mice reveal multiple roles for VEGF in skeletogenesis. Development. 2002 Apr;129(8):1893-904
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3047244
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Gene symbol:
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Vegfa
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Gene name:
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vascular endothelial growth factor A
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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The genotype of the embryos was not specified and may be either heterozygous or homozygous for the Vegfa<tm1Nagy> allele. The fixative was 0.2% glutaraldehyde, 2% paraformaldehyde.
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Results
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Specimen
4A:
embryonic day 13.5; Vegfatm1Nagy/Vegfa +
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Vegfatm1Nagy/Vegfa+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forearm |
Present |
Regionally restricted |
4A
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Expression was detected in the perichondrium and surrounding tissues of the radius and ulna with increased intensity in the area surrounding the center of the cartilage template where blood vessels will penetrate into the cartilage.
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Specimen
4B:
embryonic day 13.5; Vegfatm1Nagy/Vegfa +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Vegfatm1Nagy/Vegfa+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forearm |
Present |
Regionally restricted |
4B
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Expression was detected in the perichondrium and surrounding tissues of the radius and ulna.
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Specimen
4C:
embryonic day 13.5; Vegfatm1Nagy/Vegfa +
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Vegfatm1Nagy/Vegfa+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4D:
embryonic day 14.5; Vegfatm1Nagy/Vegfa +
(more )
Specimen
4D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Vegfatm1Nagy/Vegfa+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: vault of skull |
Present |
Regionally restricted |
4D
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The tissue was calvarium. Expression was detected in the region of mesenchyme destined to differentiate into osteoblasts.
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Specimen
4E:
embryonic day 14.5; Vegfatm1Nagy/Vegfa +
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Vegfatm1Nagy/Vegfa+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: vault of skull |
Present |
Regionally restricted |
4E
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The tissue was calvarium. Expression was detected in cells covering the surface of calvarial bone in regions where osteoid and mineral are being deposited.
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Analysis Tools
