Gene Expression Data
Assay Details
Assay
|
Reference:
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J:89092
Mitsunaga K, et al., Loss of PGC-specific expression of the orphan nuclear receptor ERR-beta results in reduction of germ cell number in mouse embryos. Mech Dev. 2004 Mar;121(3):237-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3046229
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Gene symbol:
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Esrrb
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Gene name:
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estrogen related receptor, beta
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Antibody:
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anti-ERR beta
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
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Specimen
3A:
embryonic day 7.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3B:
embryonic day 15.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3D:
embryonic day 13.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3E:
embryonic day 13.5
(more )
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3F:
embryonic day 13.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3G:
embryonic day 13.5
(more )
Specimen
3G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3H:
embryonic day 15.5
(more )
Specimen
3H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3I:
embryonic day 15.5
(more )
Specimen
3I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3J:
embryonic day 15.5
(more )
Specimen
3J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Female
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3K:
embryonic day 15.5
(more )
Specimen
3K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not Shown:
embryonic day 16.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3N:
embryonic day 14.5
(more )
Note: Double labeled: purple - Esrrb; brown - Nr5a1 (assay MGI:3046246).
Specimen
3N:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: purple - Esrrb; brown - Nr5a1 (assay MGI:3046246).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: germ cell of testis |
Present |
Single cells |
3N
|
Cells that were postitive did not stain for Nr5a1.
|
Specimen
3O:
embryonic day 14.5
(more )
Specimen
3O:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not Shown:
embryonic day 14.5
(more )
Note: Also stained for Wt1 (assay MGI:3046247).
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Also stained for Wt1 (assay MGI:3046247).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: germ cell of testis |
Present |
Single cells |
|
Cells that were postitive did not stain for Wt1.
|
|