Gene Expression Data
Assay Details
Assay
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Reference:
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J:65401
Sheth B, et al., Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mech Dev. 2000 Oct;97(1-2):93-104
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3044908
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Gene symbol:
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Rab13
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Gene name:
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RAB13, member RAS oncogene family
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Antibody:
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Rab13 antibody
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Detection system:
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Secondary antibody coupled to other - see notes
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Assay notes:
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Secondary antibody was labeled with either FITC or Alexa 488.
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Results
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Specimen
6A:
embryonic day 2.5
(more )
Note: 8-cell embryo.
Specimen
6A:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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8-cell embryo.
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Specimen
6B:
embryonic day 2.5
(more )
Note: Compact 8-cell embryo.
Specimen
6B:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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Compact 8-cell embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
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6B
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Expression is in cytoplasm and in discontinuous membrane foci along the lateral membrane and the apicolateral margin.
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Specimen
6C:
embryonic day 2.5
(more )
Note: Compact 8-cell embryo.
Specimen
6C:
(close )
Genetic Background:
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MF1
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Age:
|
embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
|
Compact 8-cell embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
|
6C
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Expression is in cytoplasm and in discontinuous membrane foci along the lateral membrane and the apicolateral margin.
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Specimen
6D,F:
embryonic day 2.5
(more )
Note: Compact 8-cell embryo. Double labeled: green - Rab13; red - Tjp1 (assay MGI:3044895).
Specimen
6D,F:
(close )
Genetic Background:
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MF1
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Age:
|
embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
|
Compact 8-cell embryo. Double labeled: green - Rab13; red - Tjp1 (assay MGI:3044895).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
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6F
,
6D
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Expression is in cytoplasm and in discontinuous membrane foci along the lateral membrane and the apicolateral margin.
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Specimen
6G,H:
embryonic day 3.0
(more )
Note: 16-cell embryo.
Specimen
6G,H:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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16-cell embryo.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Regionally restricted |
6H
,
6G
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Expression is in the outer cell lineage in the apicolateral contact sites.
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Specimen
6I,J:
embryonic day 3.5
(more )
Note: Early blastocyst.
Specimen
6I,J:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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Early blastocyst.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: trophectoderm |
Present |
Regionally restricted |
6J
,
6I
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Expression is in the apicolateral contact sites.
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Specimen
6K,L:
embryonic day 4.0
(more )
Note: Late blastocyst.
Specimen
6K,L:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 4.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
Formaldehyde
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Note:
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Late blastocyst.
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Structure |
Level |
Pattern |
Image |
Note |
TS5: trophectoderm |
Present |
Regionally restricted |
6K
,
6L
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Expression is in the apicolateral contact sites.
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