Gene Expression Data
Assay Details
Assay
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Reference:
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J:67784
Takeda S, et al., Continuous expression of Cbfa1 in nonhypertrophic chondrocytes uncovers its ability to induce hypertrophic chondrocyte differentiation and partially rescues Cbfa1-deficient mice. Genes Dev. 2001 Feb 15;15(4):467-81
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3044752
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Gene symbol:
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Col1a1
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Gene name:
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collagen, type I, alpha 1
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Probe:
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Col1a1 probe6
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: scapula cartilage condensation |
Present |
Regionally restricted |
1C
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Expression was detected in osteoblasts but was absent from prehypertrophic chondrocytes.
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Specimen
1G:
embryonic day 16.5
(more )
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: skeleton |
Present |
Regionally restricted |
1G
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Expression was detected in osteoblasts of long bones.
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Specimen
1K:
postnatal newborn
(more )
Specimen
1K:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS27: skeletal system |
Present |
Regionally restricted |
1K
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Expression was detected in osteoblasts.
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Specimen
5A:
embryonic day 16.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: rib |
Present |
Regionally restricted |
5A wt alpha1(I)
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Expression was detected in chondrocostal cartilage.
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Specimen
5B:
postnatal newborn
(more )
Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: rib |
Present |
Regionally restricted |
5B wt alpha1(I)
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Expression was detected in chondrocostal cartilage.
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