Probe:	neuroD probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: otic placode epithelium	Present	Regionally restricted	1D	Expression is located near the AP midline of the placode and coincides with that of Neurog1. Expression occurs near a border of Tbx1 expression.

Structure	Level	Pattern	Image	Note
TS14: otic placode epithelium	Present	Regionally restricted	1E	The expression domain coincides with Neurog1 and extends into the anterolateral, anteroventral and posteroventral portions of the invaginating placode. Expression overlaps with the low-level Tbx1 signal posteriorly.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	2D	A border of expression is seen at the AP midline and the posterolateral region shows no expression.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	2E	A border of expression is seen at the AP midline and the posterolateral region shows no expression. The expression is complementary to that of Tbx1.

Structure	Level	Pattern	Image	Note
TS18: otocyst epithelium	Present	Regionally restricted	2F	A border of expression is seen at the AP midline and the posterolateral region shows no expression. The expression is complementary to that of Tbx1.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	2G	There is ectopic expression posteroventromedially.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	2H	There is extopic expression posteroventromedially and anterodorsolaterally.

Structure	Level	Pattern	Image	Note
TS18: otocyst epithelium	Present	Regionally restricted	2I	There is extopic expression posteroventromedially and anterodorsolaterally.

Structure	Level	Pattern	Image	Note
TS14: otic placode epithelium	Present	Regionally restricted	2J	There is a uniform distribution of expression along the A-P axis and no AP midline border forms.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	2K	There is a uniform distribution of expression along the A-P axis and no AP midline border forms.

Structure	Level	Pattern	Image	Note
TS18: otocyst epithelium	Present	Regionally restricted	2L	There is a uniform distribution of expression along the A-P axis and no AP midline border forms.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	3I	There is a uniform distribution of expression along the A-P axis and no AP midline border forms.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	3J	A border of expression is seen at the AP midline and the posterolateral region shows no expression. The expression is complementary to that of Tbx1.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1F	There is no overlap between this expression and that of Tbx1 shown in Fig. S1A-S1E.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1G	There is no overlap between this expression and that of Tbx1 shown in Fig. S1A-S1E.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1H	There is no overlap between this expression and that of Tbx1 shown in Fig. S1A-S1E.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1I	There is no overlap between this expression and that of Tbx1 shown in Fig. S1A-S1E.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1J	There is no overlap between this expression and that of Tbx1 shown in Fig. S1A-S1E.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1K	There is an incresed accumulation of delaminated, expressing cells.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1L	There is an incresed accumulation of delaminated, expressing cells.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1M	There is an incresed accumulation of delaminated, expressing cells.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1N	There is an incresed accumulation of delaminated, expressing cells.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1O	There is an incresed accumulation of delaminated, expressing cells.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1P	The extreme dorsal and medial borders of expression seen in wild-type embryos and the signal gradient along the medial-lateral axis are mainatained in these mutants.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1Q	The extreme dorsal and medial borders of expression seen in wild-type embryos and the signal gradient along the medial-lateral axis are mainatained in these mutants.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1R	The extreme dorsal and medial borders of expression seen in wild-type embryos and the signal gradient along the medial-lateral axis are mainatained in these mutants.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1S	The extreme dorsal and medial borders of expression seen in wild-type embryos and the signal gradient along the medial-lateral axis are mainatained in these mutants.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	S1T	The extreme dorsal and medial borders of expression seen in wild-type embryos and the signal gradient along the medial-lateral axis are mainatained in these mutants.