Probe:	Grg4 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	2A	Expression is in lateral mesoderm.
TS11: extraembryonic mesoderm	Present	Regionally restricted	2A	Expression is in the migrating mesoderm.
TS11: amnion	Present		2A

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Strong	Regionally restricted	3A	Expression is in the neural plate in the primitive streak region.
TS12: future prosencephalon	Strong		3A
TS12: future midbrain	Strong	Regionally restricted	3A	Expression is in a diffuse band.
TS12: future hindbrain	Present	Regionally restricted	3A	Expression is in a diffuse band in the metencephalon and is weakly expressed in two stripes in the myelencephalon.
TS12: trunk unsegmented mesenchyme	Present		3A
TS12: trunk somite	Present	Regionally restricted	3A	Expression is in the newly formed somites and continued in the caudal half of each somite.
TS12: 1st branchial pouch	Present		3A

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	4A	Expression is in the telencephalon.
TS14: olfactory placode	Present		4A
TS14: future hindbrain	Present	Regionally restricted	4A	Expression is dorsal.
TS14: future midbrain	Present	Regionally restricted	4A	Expression is dorsal.
TS14: trunk somite	Present	Regionally restricted	4A	Expression is in the caudal part of each somite.
TS14: trunk unsegmented mesenchyme	Present		4A
TS14: neural tube	Present	Regionally restricted	4A	Expression is dorsal.
TS14: trunk mesenchyme	Present	Regionally restricted	4A	Expression is in the lateral mesoderm.
TS14: future spinal cord neural plate	Present	Regionally restricted	4A	Expression is in the open neural plate.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	5A	Expression is dorsal.
TS14: otic pit	Present		5A	Expression is in the otic vesicle.
TS14: pharyngeal region	Present	Regionally restricted	5A	Expression is in the most lateral portion of the pharynx.
TS14: pharyngeal region mesenchyme	Present		5A	Expression is in mesenchyme neighboring the pharynx.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Present		5B
TS14: trunk unsegmented mesenchyme	Present		5B
TS14: mesenchyme derived from somatopleure	Present		5B
TS14: mesenchyme derived from splanchnopleure	Present		5B

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	5C	Expression is in a dorso-lateral region.
TS14: trunk dermomyotome	Present	Regionally restricted	5C	Expression is in the myotome and is absent in the dermatome.

Structure	Level	Pattern	Image	Note
TS19: midbrain floor plate	Present	Single cells
TS19: hindbrain	Present	Regionally restricted		Expression is in a few cells in the floor of the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present		6A
TS20: spinal cord ventricular layer	Present		6A
TS20: olfactory epithelium	Present	Regionally restricted	6A	Expression is in the neural layer.

Structure	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	Present	Regionally restricted	6B	Expression is in the ventricular zone.
TS20: dorsal root ganglion	Present		6B
TS20: spinal cord ventricular layer	Present		6B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		7A
TS22: cranial ganglion	Present		7A
TS22: vibrissa	Present		7A

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		7B
TS22: cerebral cortex marginal layer	Strong	Regionally restricted	7B	Expression is in an outer layer of cells.

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present		7C
TS22: facial ganglion	Present		7C
TS22: glossopharyngeal ganglion	Present		7C
TS22: trigeminal ganglion	Present		7C

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		7D

Structure	Level	Pattern	Image	Note
TS22: dermis	Present		7E	Expression is in the basal layer of the skin.

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present		8A
TS22: dorsal root ganglion	Present		8A
TS22: lung epithelium	Present		8A
TS22: metanephros	Present		8A
TS22: gut	Present	Regionally restricted	8A	Expression is in the endoderm.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		8B

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Strong		8C
TS22: renal cortex	Present		8C

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present	Regionally restricted	9A	Expression is strongest in the infragranular layer.
TS24: cranial ganglion	Present		9A
TS24: midbrain	Present		9A
TS24: cerebellum	Present	Regionally restricted	9A	Expression is in the external granular layer.
TS24: nasal cavity epithelium	Present		9A
TS24: salivary gland	Present		9A
TS24: tooth	Present		9A
TS24: vibrissa	Present		9A
TS24: retina	Present		9A

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present	Regionally restricted	9B	Expression is strongest in the infragranular layer.
TS24: cerebral cortex ventricular layer	Absent		9B