Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pdpk1tm1Ejm
Name:	3-phosphoinositide dependent protein kinase 1; targeted mutation 1, Edward J McManus
MGI ID:	MGI:3043781
Synonyms:	PDK1(PHki)
Gene:	Pdpk1  Location: Chr17:24292654-24369898 bp, - strand  Genetic Position: Chr17, 12.23 cM, cytoband A3.3
Alliance:	Pdpk1tm1Ejm page

Germline Transmission:	Earliest citation of germline transmission: J:90081
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutation:    Other   Mutation details: In order to ablate phosphatidylinositol 3,4,5-trisphoshate, 3 codons in exon 13 encoding arginine residues 472 through 474 were engineered to instead encoded leucince. A single loxP site remained downstream of exon 14 after a floxed neo cassette used for selection was excised via cre-mediated recombination. (J:90081)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pdpk1 Mutation:	138 strains or lines available

Original:	J:90081 McManus EJ, et al., The in vivo role of PtdIns(3,4,5)P(3) binding to PDK1 PH domain defined by knockin mutation. EMBO J. 2004 May 19;23(10):2071-82
All:	2 reference(s)