Probe:	Cbfa1 cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		1A

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present	Regionally restricted	1C	Expression was detected in the periphery of cartilage primordia in the lower body.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present	Regionally restricted	1E	Expression was detected in the periphery of cartilage primordia in the lower body.

Structure	Level	Pattern	Image	Note
TS21: tibia-fibula pre-cartilage condensation	Present	Regionally restricted	2A	Expression was detected in the hypertrophic chondrocytes.
TS21: lower leg mesenchyme	Present	Regionally restricted	2A	Expression was detected in the perichondrial region covering hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: tibia cartilage condensation	Present	Regionally restricted	2C	Expression was strong in the osteoblasts in bone marrow, the bone collar covering the layers of proliferating and hypertrophic chondrocytes, bone marrow and the terminal hypertrophic chondrocytes but was weak in the resting and proliferating chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: tibia	Strong	Regionally restricted	2E	Expression was detected in the osteoblasts and terminal hypertrophic chondrocytes

Structure	Level	Pattern	Image	Note
TS24: femur	Strong	Regionally restricted	2G	Expression was detected in the osteoblasts and terminal hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS26: tibia	Present	Regionally restricted	2I	Expression was detected in hypertrophic chondrocytes.