Probe:	Collagen IIA probe1
Probe preparation:	Antisense labelled with S35 DNA
Visualized with:	Autoradiography
Assay notes:	Identical results were obtained using another oligonucleotide probe (MGI:3041979). The probe used in this assay was 3' labelled.

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	1C	Expression was detected in the prechondrocytes of cartilage anlage.
TS23: scapula	Present	Regionally restricted	1C	Expression was detected in the prechondrocytes of cartilage anlage.
TS23: upper arm rest of mesenchyme	Absent		1C	Expression was absent from the tendon.

Structure	Level	Pattern	Image	Note
TS18: branchial arch	Present		2A/C
TS18: trunk somite	Present		2A/C
TS18: 2nd branchial arch	Strong		2A/C
TS18: skeleton	Present	Regionally restricted	2A/C	Expression was detected in the primordium of basioccipital, basisphenoid and nasal bones.

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch	Present		3A/B
TS18: trunk somite	Present	Regionally restricted	3A/B	Expression was detected in the cervical somites.
TS18: skeleton	Present	Regionally restricted	3A/B	Expression was detected in the clivus and structures that will become the cartilage primordium of the cranium.

Structure	Level	Pattern	Image	Note
TS18: otocyst epithelium	Present		4A/B
TS18: otocyst mesenchyme	Present		4A/B

Structure	Level	Pattern	Image	Note
TS26: inner ear	Present		5A/B
TS26: chondrocranium	Present	Regionally restricted	5A/B	Expression was detected in the calvaria cartilage primordia.
TS26: supraoccipital cartilage condensation	Present	Regionally restricted	5A/B	Expression was detected in the chondroprogenitor cells at the lower tip and outer side of the bone.

Structure	Level	Pattern	Image	Note
TS26: labyrinth epithelium	Present		6A/B	Expression was detected in the epithelial cells of the membranous labyrinth.
TS26: otic cartilage	Present	Regionally restricted	6A/B	Expression was detected in the chondroprogenitor cells adjacent to the cartilage of the osseus labyrinth primordium.

Structure	Level	Pattern	Image	Note
TS26: C3 vertebra	Present	Regionally restricted	7A/B	Expression was detected in the chondroprogenitor cells.
TS26: C4 vertebra	Present	Regionally restricted	7A/B	Expression was detected in the chondroprogenitor cells.
TS26: C5 vertebra	Present	Regionally restricted	7A/B	Expression was detected in the chondroprogenitor cells.

Structure	Level	Pattern	Image	Note
TS26: vertebra	Present		8A/B
TS26: nasal capsule	Present	Regionally restricted	8A/B	Expression was detected in the nasal cartilage.
TS26: mandible	Present		8A/B
TS26: sternum	Present		8A/B
TS26: trachea	Present		8A/B

Structure	Level	Pattern	Image	Note
TS26: mandible	Present	Regionally restricted	9A/B	Expression was detected in the mesenchymal cells.