Antibody:	anti-SSEA-1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		2A
TS9: extraembryonic visceral endoderm	Present		2A
TS9: trophectoderm	Present	Regionally restricted	2A	Staining is present in the trophoblastic cells invading the maternal decidua.
TS9: ectoplacental cone	Absent		2A
TS9: extraembryonic ectoderm	Absent		2A
TS9: parietal endoderm	Absent		2A
TS28: decidua	Absent		2A

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		2B
TS11: extraembryonic visceral endoderm	Present		2B , 2C
TS11: trophectoderm	Present	Regionally restricted	2B	Staining is present in the trophoblastic cells.
TS11: ectoplacental cone	Absent		2B
TS11: embryo mesoderm	Absent		2B
TS11: extraembryonic ectoderm	Absent		2B
TS11: parietal endoderm	Absent		2B
TS11: primordial germ cell	Present		2B , 2C

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		3A
TS13: foregut diverticulum endoderm	Present		3A
TS13: head mesenchyme	Absent		3A
TS13: trunk somite	Absent		3A
TS13: heart	Absent		3A	There is no staining in the primitive heart.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		3B
TS13: somatopleure	Present		3B	Staining is present in cells lining the somatopleura.
TS13: extraembryonic component	Present		3B	Staining is present in the visceral endoderm and trophoblastic cells.
TS13: yolk sac	Present		3B

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4
TS15: notochord	Present		4
TS15: gut	Present		4	Staining is present in the primitive gut.
TS15: surface ectoderm	Present	Regionally restricted	4	Staining is present in the ectodermal cells of the epidermis.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present			Staining is present in cells forming the thickened ectoderm overlying the head region.
TS17: gonad primordium	Present			Staining is present in the genital ridge.
TS17: brain	Present			Staining is present in unidentified areas of the brain.
TS17: ear	Present			Staining is present in the developing auditory vesicle.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	5a/b	Staining is present in the genital ridges.
TS19: mesonephros	Weak		5a/b	Staining is present in the primordia of the mesonephric and Mullerian ducts.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Patchy		Staining is present in the metanephros and a few tubular cells.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of male	Present		6	Staining is present in the gonadal cells.
TS21: nephric duct of male, mesonephric portion	Present	Regionally restricted	6	Staining is present in cells lining the epididymal ducts.

Structure	Level	Pattern	Image	Note
TS23: testis	Trace		7	Almost no staining is seen in the testis.
TS23: epididymis	Strong		7	Staining is seen in the epididymal ductal cells.

Structure	Level	Pattern	Image	Note
TS24: medullary renal tubule	Present	Regionally restricted	8
TS24: renal convoluted tubule	Present	Regionally restricted	8
TS24: maturing glomerular tuft	Absent		8

Structure	Level	Pattern	Image	Note
TS28: endometrium	Strong	Regionally restricted	9A , 9B	Staining is seen on the endometrial glandular cells.

Structure	Level	Pattern	Image	Note
TS28: oviduct	Present	Patchy	10	Staining is present in some (but not all) epithelial lining cells.

Structure	Level	Pattern	Image	Note
TS28: epididymis	Present	Patchy	11	Staining is present in some (but not all) epithelial lining cells.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Weak	Regionally restricted		There is weak staining in the kidney tubular cells.
TS28: cerebellum	Weak
TS28: intestine	Absent
TS28: musculature	Absent
TS28: ovary	Absent
TS28: skin	Absent

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: spermatozoon	Present	Regionally restricted		There is staining in the epididymal (but not testicular) sperm.
TS28: ductus deferens	Absent
TS28: testis	Absent