Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73681
Saika S, et al., TGFbeta2 in corneal morphogenesis during mouse embryonic development. Dev Biol. 2001 Dec 15;240(2):419-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3041492
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Gene symbol:
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Kera
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Gene name:
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keratocan
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Probe:
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Keratocan probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The data not shown was reported in Table 1.
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Results
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Specimen
5B:
embryonic day 16.5; Tgfb2tm1Doe/Tgfb2 +
(more  )
Specimen
5B:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2+
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea stroma |
Strong |
Regionally restricted |
5B
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Expression was detected in the keratocytes.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2tm1Doe
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cornea stroma |
Weak |
Regionally restricted |
5C
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Expression was weak in the keratocytes.
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Specimen
not shown:
embryonic day 12.5; Tgfb2tm1Doe/Tgfb2 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2tm1Doe
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 14.5; Tgfb2tm1Doe/Tgfb2 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2tm1Doe
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 15.5; Tgfb2tm1Doe/Tgfb2 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2tm1Doe
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 18.5; Tgfb2tm1Doe/Tgfb2 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * Black Swiss
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Mutant Allele(s):
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Tgfb2tm1Doe/Tgfb2tm1Doe
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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