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Gene Expression Data
Assay Details
Assay
Reference: J:55558 Joester A, et al., Evidence for combinatorial variability of tenascin-C isoforms and developmental regulation in the mouse central nervous system. J Biol Chem. 1999 Jun 11;274(24):17144-51
Assay type: RT-PCR
MGI Accession ID: MGI:3039916
Gene symbol: Tnc
Gene name: tenascin C
Probe: Tnc-5forw, Tnc-6rev
Assay notes: Southern blot of PCR products was hybridized with a fluorescein-labeled probe for the specific alternatively-spliced FNIII domain A2 (MGI:3139879).
Results
Image: 9A2 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
E13 E13.5 (a) TS21: brain Present Trace 4 µg; total RNA Not Specified Not Specified
E16 E16.5 (a) TS24: brain Present Trace 4 µg; total RNA Not Specified Not Specified
P0 P0 TS27: brain Strong (d) Trace 4 µg; total RNA Not Specified Not Specified Authors state that overall expression of isoforms peaked around birth.
P7 P7 TS28: brain Present Trace 4 µg; total RNA Not Specified Not Specified
P14 P14 TS28: brain Not Specified Trace 4 µg; total RNA Not Specified Not Specified Isoform expression decreased substantially at the end of the second postnatal week.
adult P adult TS28: brain Absent Absent 4 µg; total RNA Not Specified Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Corresponding to 2-6 FNIII domains. For five of the six bands, low signal intensity in early and late development, with high intensity at P0.
(c) Corresponding to one FNIII domain. Only extremely weak signals were obtained.
(d) The two FNIII domain product developed the strongest signal at P0.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory