Lifr Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:31858 Owczarek CM, et al., Molecular basis of the soluble and membrane-bound forms of the murine leukemia inhibitory factor receptor alpha-chain. Expression in normal, gestating, and leukemia inhibitory factor nullizygous mice. J Biol Chem. 1996 Mar 8;271(10):5495-504
Assay type:	Northern blot
MGI Accession ID:	MGI:3039513
Gene symbol:	Lifr
Gene name:	LIF receptor alpha

Probe:	mLIFR cDNA1
Probe preparation:	Double stranded DNA
Visualized with:	Phosphorimaging

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	10 kb (a)	3 kb (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
non-pregnant	P w 6-8	TS28: liver	Present (b)	Present (b)	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
pseudopregnant	P w 6-8	TS28: liver	Present (b)	Present (b)	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
4 Days	P w 6-8	TS28: liver	Present	Present	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
8 Days	P w 6-8	TS28: liver	Strong (d)	Strong	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
12 Days	P w 6-8	TS28: liver	Strong (d)	Strong	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
15 Days	P w 6-8	TS28: liver	Present	Present	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
19 Days	P w 6-8	TS28: liver	Present	Present	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female
21 Days	P w 6-8	TS28: liver	Present	Present	3 µg; poly-A+ RNA	(C57BL/6 x C57BL/10)F1		Female

Notes:
(a) Authors state that the two highest molecular weight membrane-bound isoforms were analyzed as a single band of approximately 10 kb.
(b) No difference in transcript levels between normal and pseudopregnant mice.
(c) The 3 kb band encoding the soluble receptor increased sharply (approximately 20-fold) around day 8 of pregnancy, peaked at day 12, and dropped to relatively low levels at day 21.
(d) Levels were maximal around days 8-12 of pregnancy (increased approximately 10-fold).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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