Reporter:	lacZ
	Direct Detection
Assay notes:	Fixation was with 2% paraformaldehyde and 2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Uniform		Authors report diffuse staining throughout whole embryonic portion of egg cylinder.
TS9: extraembryonic component	Absent			No expression in extraembryonic tissues, including yolk sac, extraembryonic ectoderm and ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	1A	Strongest staining along midline of neural plate and in forming head fold. Diffuse staining also visible throughout rest of neural plate.
TS11: primitive streak	Present		1A
TS11: extraembryonic component	Absent		1A	No expression in extraembryonic tissues, including yolk sac, extraembryonic ectoderm and ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Uniform	1B-D	Authors report diffuse staining throughout embryo.
TS13: future brain	Present	Regionally restricted	1B-D	Intense staining in anteriormost aspect of brain (prosencephalon) and continued caudally through mesencephalon, rhombencephalon and fusing neural folds.
TS13: future spinal cord	Present	Regionally restricted	1B-D	Intense staining in anteriormost aspect of brain (prosencephalon) and continued caudally through mesencephalon, rhombencephalon and fusing neural folds.
TS13: neural tube lateral wall	Present		1B-D	Neural tube staining restricted to lateral sides.
TS13: neural tube floor plate	Absent		1B-D
TS13: branchial arch	Present		1B-D
TS13: heart	Present		1B-D
TS13: notochord	Present		1B-D
TS13: primitive streak	Present		1B-D
TS13: extraembryonic component	Absent		1B-D	No expression in extraembryonic tissues, including yolk sac, extraembryonic ectoderm and ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Uniform	1E	Diffuse staining throughout whole embryo.
TS15: future brain	Present	Regionally restricted	1E
TS15: 1st branchial arch	Strong		1E	Authors report darker staining in telencephalon, neural tube, heart, and first and second branchial arches.
TS15: 2nd branchial arch	Strong		1E	Authors report darker staining in telencephalon, neural tube, heart, and first and second branchial arches.
TS15: heart	Strong		1E	Authors report darker staining in telencephalon, neural tube, heart, and first and second branchial arches.
TS15: neural tube	Strong		1E	Authors report darker staining in telencephalon, neural tube, heart, and first and second branchial arches.
TS15: telencephalon	Strong		1E	Authors report darker staining in telencephalon, neural tube, heart, and first and second branchial arches.
TS15: eye	Present		1E
TS15: forelimb bud	Present		1E
TS15: extraembryonic component	Absent		1E	No expression in extraembryonic tissues, including yolk sac, extraembryonic ectoderm and ectoplacental cone.