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Gene Expression Data
Assay Details
Assay
Reference: J:31258 Kozmik Z, et al., Alternative splicing of Pax-8 gene transcripts is developmentally regulated and generates isoforms with different transactivation properties. Mol Cell Biol. 1993 Oct;13(10):6024-35
Assay type: RNase protection
MGI Accession ID: MGI:3038303
Gene symbol: Pax8
Gene name: paired box 8
Probe: Pax8 probe3
Probe preparation: RNA
Visualized with: Autoradiography
Results Image: 9
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
E9.5 we E9.5 (a) TS15: conceptus Present Present 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E9.5 ep E9.5 (a) TS15: embryo Not Specified Present (d) 20 µg; total RNA Not Specified Not Specified embryo proper
E9.5 m E9.5 (a) TS15: extraembryonic component Absent (e) Absent (e) 20 µg; total RNA Not Specified Not Specified extraembryonic membranes; visceral yolk sac and amnion
E9.5 p E9.5 (a) TS28: placenta Present (f) Not Specified 20 µg; total RNA Not Specified Not Specified
E10.5 we E10.5 (a) TS17: conceptus Present Present 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E10.5 ep E10.5 (a) TS17: embryo Not Specified Present (g) 20 µg; total RNA Not Specified Not Specified embryo proper
E10.5 m E10.5 (a) TS17: extraembryonic component Absent (e) Absent (e) 20 µg; total RNA Not Specified Not Specified extraembryonic membranes; visceral yolk sac and amnion
E10.5 p E10.5 (a) TS28: placenta Present Not Specified 20 µg; total RNA Not Specified Not Specified
E11.5 we E11.5 (a) TS19: conceptus Present Present 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E11.5 ep E11.5 (a) TS19: embryo Not Specified Present (g) 20 µg; total RNA Not Specified Not Specified embryo proper
E11.5 m E11.5 (a) TS19: extraembryonic component Absent (e) Absent (e) 20 µg; total RNA Not Specified Not Specified extraembryonic membranes; visceral yolk sac and amnion
E11.5 p E11.5 (a) TS28: placenta Present Not Specified 20 µg; total RNA Not Specified Not Specified
E12.5 we E12.5 (a) TS20: conceptus Present Present 20 µg; total RNA Not Specified Not Specified embryo proper plus extraembryonic membranes plus placenta
E12.5 ep E12.5 (a) TS20: embryo Not Specified Present (h) 20 µg; total RNA Not Specified Not Specified embryo proper
E12.5 m E12.5 (a) TS20: extraembryonic component Absent (e) Absent (e) 20 µg; total RNA Not Specified Not Specified extraembryonic membranes; visceral yolk sac and amnion
E12.5 p E12.5 (a) TS28: placenta Present (i) Not Specified 20 µg; total RNA Not Specified Not Specified
M Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Transcripts a, e and f; predominantly expressed in placenta.
(c) Transcripts c, b and d; coexpressed in the embryo proper.
(d) At day 9.5, b and d are expressed at lower level relative to a.
(e) No Pax-8 transcripts were detectable in extraembryonic membranes.
(f) Expression of e steadily declines starting with day 9.5.
(g) Expression of isoforms b and d increases.
(h) At day 12.5, b and d are expressed at the same abundance as a.
(i) At day 12.5, a is the only prevalent form expressed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory