Probe:	Periostin cDNA1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Absent		2A
TS10: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS28: endometrium	Present	Single cells	2A	Expression was detected in decidualized endometrial stromal cells.
TS28: myometrium	Present		2A

Structure	Level	Pattern	Image	Note
TS14: extraembryonic component	Present	Regionally restricted	2C , 2B	Expression was detected in the amnion.
TS14: extraembryonic vascular system	Present	Regionally restricted	2B , 2C	Expression in the vitelline artery.
TS14: atrioventricular canal	Absent		2C , 2B
TS14: embryo	Absent		2C , 2B
TS14: heart	Absent		2C , 2B
TS14: neural tube	Absent		2C , 2B
TS14: outflow tract	Absent		2C , 2B

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Single cells	2D
TS15: blood vessel	Strong	Regionally restricted	2D	Expression was detected in the umbilical vessels.
TS15: blood	Absent		2D	Expression was absent from blood cells.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		2E
TS17: dorsal root ganglion	Present		2E
TS17: heart	Present		2E
TS17: extraembryonic component	Present	Regionally restricted	2E	Expression was detected in the amnion.
TS17: blood vessel	Present	Regionally restricted	2E	Expression was detected in umbilical vessels.
TS17: endocardial cushion	Strong	Regionally restricted	2E , 2F	Expression was detected in the endocardial cushions of the conotruncus (only in the mesenchymal cushion cells).
TS17: atrioventricular canal	Strong		2E , 2F
TS17: embryo mesenchyme	Present	Regionally restricted	2E , 2F	Expression was detected in the epicardial mesenchyme overlying the heart.
TS17: cardiac muscle tissue	Absent		2F	Expression was not detected within either inducing myocardial cells or responding endocardial cells.
TS17: endocardial lining	Absent		2F	Expression was not detected within either inducing myocardial cells or responding endocardial cells.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Strong		2G
TS20: embryo mesenchyme	Strong	Regionally restricted	2G	Expression was detected in the mesenchyme surrounding the aortic arch and pulmonary trunk.
TS20: endocardial cushion	Strong	Regionally restricted	2G	Expression was detected in the conotruncal cushion.
TS20: cardiac muscle tissue	Absent		2G	Expression was not detected within either inducing myocardial cells or responding endocardial cells.
TS20: endocardial lining	Absent		2G	Expression was not detected within either inducing myocardial cells or responding endocardial cells.

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong	Regionally restricted	3A	Expression was detected surrounding the aorta at the level where the ductus arteriosus meets the aorta.
TS22: embryo	Present		3A
TS22: pulmonary valve	Strong	Regionally restricted	3A	Expression was detected in the pulmonary valve leaflet.
TS22: tricuspid valve	Strong		3A

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Weak	Regionally restricted	3B	Expression was detected in the wall of the aorta.
TS22: pulmonary valve	Strong	Regionally restricted	3B	Expression was detected in the pulmonary valve leaflets.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Strong		3C
TS22: tricuspid valve	Strong		3C
TS22: left atrium cardiac muscle	Weak	Spotted	3C
TS22: left ventricle cardiac muscle	Weak	Spotted	3C
TS22: right atrium cardiac muscle	Weak	Spotted	3C
TS22: right ventricle cardiac muscle	Weak	Spotted	3C

Structure	Level	Pattern	Image	Note
TS25: tricuspid valve	Strong		3E , 3F , 3D
TS25: cardiac muscle tissue	Weak		3E , 3F , 3D

Structure	Level	Pattern	Image	Note
TS25: rib	Strong	Regionally restricted	3G	Expression was detected in the periosteum of the rib primordium.

Structure	Level	Pattern	Image	Note
TS28: mitral valve	Present		3I , 3H
TS28: aorta	Present	Regionally restricted	3H , 3I	Expression was detected in the aorta wall.

Structure	Level	Pattern	Image	Note
TS28: heart	Present		3K , 3J
TS28: cardiac muscle tissue	Weak		3J , 3K