Antibody:	Anti-gamma catenin/plakoglobin
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Identical results were obtained with another antibody (MGI:3033870).

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Weak	Regionally restricted	3A	Expression was detected at the subsarcolemmal level and at the surface of the myotubes in hindlimb muscle.
TS20: blood vessel	Present		3A

Structure	Level	Pattern	Image	Note
TS22: hindlimb musculature	Present	Regionally restricted	3B	Expression was detected at sites of contact between myotubes and was strong in the sarcoplasm.
TS22: blood vessel	Present		3B

Structure	Level	Pattern	Image	Note
TS24: hindlimb musculature	Present	Regionally restricted	3C	Expression was detected at sites of contact between myotubes and was strong in the sarcoplasm.
TS24: blood vessel	Present		3C

Structure	Level	Pattern	Image	Note
TS26: hindlimb musculature	Present	Patchy	3D	Expression was detected in sarcoplasm of some fibers.
TS26: blood vessel	Present		3D

Structure	Level	Pattern	Image	Note
TS27: gastrocnemius muscle	Present	Patchy	3E	Expression was detected in sarcoplasm of some fibers.
TS27: blood vessel	Present		3E

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Patchy	3F	Expression was detected in sarcoplasm (at lower levels than at previous stages) of some fibers.
TS28: blood vessel	Present		3F

Structure	Level	Pattern	Image	Note
TS24: hindlimb musculature	Present	Regionally restricted	4C	Expression was detected in the sarcoplasm and at sites of contact between primary and secondary myotubes.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Regionally restricted	6C	Expression was detected at synaptic sites in the axoplasm of terminal nerve branches on the motor endplate.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Regionally restricted	7C	Expression was detected in the intramuscular nerves but was almost undetectable in the muscle fibers.
TS28: blood vessel	Present		7C