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Gene Expression Data
Assay Details
Assay
Reference: J:10576 Rosner MH, et al., A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature. 1990 Jun 21;345(6277):686-92
Assay type: RNA in situ
MGI Accession ID: MGI:3033439
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Probe: Oct3 Probe2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 5a/b: postnatal adult (more )
Note: Oocyte.
Structure Level Pattern Image Note
TS28: primary oocyte Present 5a/b
Specimen not shown: postnatal adult (more )
Note: Unfertilized Ova.
Structure Level Pattern Image Note
TS28: secondary oocyte Present
Specimen 5c/d: embryonic day 2.5 (more )
Note: Morula.
Structure Level Pattern Image Note
TS3: compacted morula Present 5c/d
Specimen 5e/f: embryonic day 3.5 (more )
Note: Blastocyst.
Structure Level Pattern Image Note
TS4: trophectoderm Present 5e/f
TS4: inner cell mass Strong 5e/f
Specimen 5g/h: embryonic day 4.5 (more )
Note: Hatched Blastocyst.
Structure Level Pattern Image Note
TS6: trophectoderm Absent 5g/h
TS6: epiblast Strong 5g/h
TS6: primitive endoderm Moderate 5g/h
Specimen 5i: embryonic day 5.5 (more )
Note: Egg cylinder stage embryo.
Structure Level Pattern Image Note
TS7: epiblast Present Regionally restricted 5i Author states that expression is seen in the primitive ectoderm.
TS7: extraembryonic visceral endoderm Absent 5i
TS7: trophectoderm Absent 5i
Specimen 5j/k: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo mesoderm Weak Regionally restricted 5j/k Authors state that expression is detected in primitive ectoderm-derived mesoderm.
TS11: embryo ectoderm Present 5j/k
TS11: extraembryonic visceral endoderm Absent 5j/k
Specimen 5l/m: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo mesoderm Weak Regionally restricted 5l/m Authors state that expression is detected in primitive ectoderm-derived mesoderm.
TS11: embryo ectoderm Present 5l/m
TS11: extraembryonic visceral endoderm Absent 5l/m
Specimen 5n/o: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: embryo ectoderm Present 5n/o
Specimen 5p/r: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of hindgut Present 5p , 5r Expression in migrating primordial germ cells.
Specimen 5t: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5t Expression in primordial germ cells in the genital ridges.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
MGI 6.24 		The Jackson Laboratory