Probe:

Msal-1 cDNA

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Graded	2B	Expression is in a caudal to rostral gradient.
TS11: allantois	Present		2B
TS11: head mesenchyme	Present	Regionally restricted	2B	Expression is in the pre-somitic mesenchyme, absent in the headfold mesoderm.
TS11: primitive streak	Strong		2B

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	2D	Expression is ventral.
TS12: neural tube	Present	Regionally restricted	2D	Expression is ventral.
TS12: embryo	Present	Regionally restricted	2D	Expression is in the tail bud.
TS12: 1st branchial arch mesenchyme derived from neural crest	Present		2D

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present		3A
TS16: future hindbrain	Present	Regionally restricted	3A	Expression is at the midbrain/hindbrain boundary.
TS16: future midbrain	Present	Regionally restricted	3A	Expression is at the midbrain/hindbrain boundary.
TS16: neural tube	Present		3A
TS16: limb	Present		3A
TS16: oral region	Present	Regionally restricted	3A	Expression is in the palate.
TS16: facio-acoustic ganglion complex	Present		3A
TS16: glossopharyngeal ganglion	Present		3A
TS16: glossopharyngeal-vagus ganglion complex	Present		3A
TS16: otocyst	Present		3A
TS16: trigeminal ganglion	Present		3A
TS16: vagus ganglion	Present		3A
TS16: mesonephric tubule	Present		3A	Expression is in the pronephric and mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Strong	Regionally restricted	3C	Expression is in the ventral forebrain.
TS16: diencephalon	Strong		3C
TS16: future hindbrain	Strong		3C
TS16: otocyst	Weak	Regionally restricted	3C	Expression is in the ventral otic vesicle.
TS16: oral region	Present	Regionally restricted	3C	Expression is in the palate.
TS16: facio-acoustic ganglion complex	Present		3C
TS16: glossopharyngeal ganglion	Present		3C
TS16: trigeminal ganglion	Present		3C
TS16: vagus ganglion	Present		3C

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Weak		3F
TS17: notochord	Present		3F
TS17: neural tube lateral wall	Absent		3F	Expression is absent from dorsal ventricular zone cells.
TS17: neural tube floor plate	Absent		3F

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present		4A
TS20: alar columns	Strong		4A
TS20: basal columns	Present	Single cells	4A	Expression is some differentiating neurons.
TS20: notochord	Absent		4A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		4C	Expression is in the intermediate and ventral ventricular zone cells.
TS20: hypothalamus	Present	Regionally restricted	4C	Expression is in the ventral neurons.
TS20: telencephalon	Present	Regionally restricted	4C	Expression is in dorso-lateral telencephalic cells.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present		4E	Expression is in the ganglionic eminence.
TS20: brain	Present	Single cells	4E	Expression is in differentiating neurons in the pre-optic area.
TS20: hindbrain	Present	Single cells	4E	Expression is in motor neuron nuclei.

Structure	Level	Pattern	Image	Note
TS21: renal cortex	Weak	Regionally restricted	5A	Expression is in the glomeruli.

Structure	Level	Pattern	Image	Note
TS21: heart	Strong	Regionally restricted	5B	Expression is in the cushion pads.

Structure	Level	Pattern	Image	Note
TS21: limb digit	Weak
TS21: limb interdigital region	Weak