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Gene Expression Data
Assay Details
Assay
Reference: J:39665 Pleau JM, et al., Ontogeny of glutamic acid decarboxylase gene expression in the mouse pancreas. Biochem Biophys Res Commun. 1997 Apr 7;233(1):227-30
Assay type: RT-PCR
MGI Accession ID: MGI:3029868
Gene symbol: Gad1
Gene name: glutamate decarboxylase 1
Probe: GAD67-sense, GAD67-antisense
Probe preparation: labelled with P32 DNA
Visualized with: Ethidium bromide
Assay notes: The samples were amplified in the presence of a radioactive nucleotide and were quantified in FIG. 2.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
-2 male E18.0 (a) TS26: pancreas Moderate 3 µg; total RNA NOD Male
-2 female E18.0 (a) TS26: pancreas Moderate 3 µg; total RNA NOD Female
0 male P newborn TS27: pancreas Moderate 3 µg; total RNA NOD Male
0 female P newborn TS27: pancreas Moderate 3 µg; total RNA NOD Female
14 male P14 TS28: pancreas Moderate 3 µg; total RNA NOD Male
14 female P14 TS28: pancreas Moderate 3 µg; total RNA NOD Female
21 male P21 TS28: pancreas Moderate 3 µg; total RNA NOD Male
21 female P21 TS28: pancreas Moderate 3 µg; total RNA NOD Female
35 male P35 TS28: pancreas Strong 3 µg; total RNA NOD Male
35 female P35 TS28: pancreas Strong 3 µg; total RNA NOD Female
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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