Probe:	Vrho cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural fold	Present		2A

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural fold	Present		2B

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		2C
TS13: 1st branchial arch	Present	Regionally restricted	2C	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS14: future midbrain roof plate	Present	Regionally restricted	2D	Expression is in the prospective pretectum.
TS14: 1st branchial arch	Present		2D	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch ectoderm	Present

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present		2E	Expression is in the caudal part of the last formed somites.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	3A	Expression is in the ventral forebrain.
TS14: future diencephalon	Strong	Regionally restricted	3A	Expression is dorsal.
TS14: future midbrain roof plate	Strong	Regionally restricted	3A	Expression is in the prospective pretectum.
TS14: future hindbrain	Present	Regionally restricted	3A	Expression is in the metencephalon.
TS14: 1st branchial arch	Present		3A

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	3B	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Graded	3C	Expression is less prominent in the ontogenetically younger more caudal part of the tube.
TS14: hindgut diverticulum	Strong		3C

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	3D	Expression is less prominent in the ontogenetically younger more caudal part of the tube.
TS15: tail neural tube	Present	Graded	3D	Expression is less prominent in the ontogenetically younger more caudal part of the tube.
TS15: hindgut diverticulum	Strong		3D
TS15: otocyst	Present		3D
TS15: diencephalon	Strong		3D
TS15: future midbrain roof plate	Strong	Regionally restricted	3D	Expression is in the prospective pretectum.
TS15: future hindbrain	Present	Regionally restricted	3D	Expression is in the ventral metencephalon.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Strong		3E
TS15: diencephalon	Strong		3E
TS15: future midbrain roof plate	Strong	Regionally restricted	3E	Expression is in the prospective pretectum.
TS15: future hindbrain	Present	Regionally restricted	3E	Expression is in the ventral metencephalon.
TS15: 1st branchial arch	Present		3E
TS15: trigeminal preganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3F

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Graded	3G	Expression is less prominent in the ontogenetically younger more caudal part of the tube.
TS16: tail future spinal cord	Present	Graded	3G	Expression is less prominent in the ontogenetically younger more caudal part of the tube.
TS16: hindgut diverticulum	Strong		3G
TS16: otocyst	Present		3G
TS16: future forebrain	Present	Regionally restricted	3G	Expression is ventral.
TS16: diencephalon	Strong		3G
TS16: future midbrain roof plate	Strong	Regionally restricted	3G	Expression is in the prospective pretectum.
TS16: future hindbrain	Present	Regionally restricted	3G	Expression is in the ventral metencephalon.
TS16: 1st branchial arch	Present		3G
TS16: facio-acoustic ganglion complex	Present		3G

Structure	Level	Pattern	Image	Note
TS16: embryo	Present		4A

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	4B	Expression is in the pretectum at both sides of the ventral midline extending into the alar plate.
TS16: future midbrain roof plate	Present	Regionally restricted	4B	Expression is in the pretectum at both sides of the ventral midline extending into the alar plate.
TS16: diencephalon	Present		4B

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	4C	Expression in the metencephalon is ventral.

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present		4D
TS16: future hindbrain	Present	Regionally restricted	4D	Expression is absent in the ventral rhombencephalon at the level of the otic vesicles, although it is present elsewhere in the rhombencephalon.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Weak	Regionally restricted	4E	Expression is at the transition of the rhombencephalon and the spinal neural tube.
TS16: neural tube	Weak	Regionally restricted	4E	Expression is at the transition of the rhombencephalon and the spinal neural tube.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Strong		4F	This section is at the level of the forelimbs. Expression is in two longitudinal stripes along both sides of the ventral midline. There is a second weak dorsal expression domain.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Graded	4G	Expression decreases towards the ontogenetically younger more caudal parts of the neural tube. Expression is in two longitudinal stripes along both sides of the ventral midline.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Graded	4H	Expression decreases towards the ontogenetically younger more caudal parts of the neural tube. Expression is in two longitudinal stripes along both sides of the ventral midline.
TS16: hindgut diverticulum	Present		4H

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	4I	This section is at the most caudal part of the neural tube, where expression is absent; more rostral parts do contain expression.
TS16: hindgut diverticulum	Present		4I