Kras Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:49116 Sithanandam G, et al., Selective mutation of K-ras by N-ethylnitrosourea shifts from codon 12 to codon 61 during fetal mouse lung maturation. Oncogene. 1998 Jul 30;17(4):493-502
Assay type:	Western blot
MGI Accession ID:	MGI:3029213
Gene symbol:	Kras
Gene name:	Kirsten rat sarcoma viral oncogene homolog

Antibody:	anti-c-K-ras
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The protein on the Western was isolated by immunoprecipitation with a different antibody (MGI:3029199) prior to loading on SDS-PAGE.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex
14	E13.5 (a)	TS21: lung	Weak	BALB/c		Pooled
14	E13.5 (a)	TS21: lung	Weak	BALB/c		Pooled
16	E15.5 (a)	TS23: lung	Present	BALB/c		Pooled
16	E15.5 (a)	TS23: lung	Present	BALB/c		Pooled
16	E15.5 (a)	TS23: lung	Present	BALB/c		Pooled
18	E17.5 (a)	TS25: lung	Present	BALB/c		Pooled
18	E17.5 (a)	TS25: lung	Present	BALB/c		Pooled
18	E17.5 (a)	TS25: lung	Present	BALB/c		Pooled
Adult	P w 6	TS28: lung	Present	BALB/c		Male
Adult	P w 6	TS28: lung	Present	BALB/c		Male
Adult	P w 6	TS28: lung	Present	BALB/c		Male

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels increased progressively in E15.5 and E17.5 (2.8-fold increase from E13.5 to E17.5) and peaked in adult (1.5-2.2-fold increase from E17.5 to adult).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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