Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:86666
Michno K, et al., Shh expression is required for embryonic hair follicle but not mammary gland development. Dev Biol. 2003 Dec 1;264(1):153-65
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3028998
|
|
Gene symbol:
|
Lef1
|
|
Gene name:
|
lymphoid enhancer binding factor 1
|
|
Antibody:
|
anti-Lef1
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
6A:
embryonic day 14.5
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mammary gland primordium |
Present |
Regionally restricted |
6B
|
Expression is in most cells. Expression in this mutant is the same as in wild-type embryos.
|
| TS22: mammary mesenchyme |
Present |
Regionally restricted |
6B
|
Expression is in the condensed cells. Expression in this mutant is the same as in wild-type embryos.
|
Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: integumental system |
Present |
Regionally restricted |
6C
|
Expression is in most epithelial cells and the condensing mesenchymal cells underlying presumptive hair follicles.
|
Specimen
6D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: integumental system |
Present |
Regionally restricted |
6D
|
Expression is in most epithelial cells and the condensing mesenchymal cells underlying presumptive hair follicles. Expression in this mutant is the same as in wild-type embryos.
|
Specimen
9A:
embryonic day 18.5
(more )
Specimen
9A:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
9A
|
Expression is in epithelial and mesenchymal cells.
|
Specimen
9B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
9B
|
Expression is in epithelial and mesenchymal cells. Expression in this mutant is the same as in wild-type embryos.
|
Specimen
9C:
embryonic day 18.5
(more )
Specimen
9C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
9D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mammary gland epithelium |
Present |
Regionally restricted |
9D
|
Expression is in most cells. Expression in this mutant is the same as in wild-type embryos.
|
| TS26: mammary mesenchyme |
Present |
Regionally restricted |
9D
|
Expression is in most cells. Expression in this mutant is the same as in wild-type embryos.
|
|
Analysis Tools