Dab1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:54100 Howell BW, et al., Reelin-induced tyrosine phosphorylation of disabled 1 during neuronal positioning. Genes Dev. 1999 Mar 15;13(6):643-8
Assay type:	Western blot
MGI Accession ID:	MGI:3028532
Gene symbol:	Dab1
Gene name:	disabled 1

Antibody:

anti-mDab1 B3

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex	Note
wt	Control
wt	E16.0 (a)	TS24: brain	Present	Not Specified		Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
-/+	E16.0 (a)	TS24: brain	Present	B6C3Fe-a/a	Reln^rl/Reln⁺	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
rl	E16.0 (a)	TS24: brain	Present (b)	B6C3Fe-a/a	Reln^rl/Reln^rl	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
wt	Control
wt	P21	TS28: brain	Not Specified	Not Specified		Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
-/+	P21	TS28: brain	Not Specified	B6C3Fe-a/a	Reln^rl/Reln⁺	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
rl	P21	TS28: brain	Not Specified	B6C3Fe-a/a	Reln^rl/Reln^rl	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
wt	E16.0 (a)	TS24: brain	Present	Not Specified		Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
rl	E16.0 (a)	TS24: brain	Present (c)	B6C3Fe-a/a	Reln^rl/Reln^rl	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.
rl	E16.0 (a)	TS24: brain	Present (d)	B6C3Fe-a/a	Reln^rl/Reln^rl	Not Specified	The sample was immunoprecipitated with the same antibody prior to Western blotting.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was elevated in the mutant at E16.
(c) The sample was diluted (.2) to estimate relative protein levels.
(d) The sample was diluted (.5) to estimate relative protein levels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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