Probe:	Rgma cDNA
Assay notes:	All sections are vibratome sections.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present		2A

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		2D

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2G	There are gaps in expression at the mid-hindbrain and the fore-midbrain boundaries.
TS15: future hindbrain	Present	Regionally restricted	2G	There are gaps in expression at the mid-hindbrain and the fore-midbrain boundaries.
TS15: future midbrain	Present	Regionally restricted	2G	There are gaps in expression at the mid-hindbrain and the fore-midbrain boundaries.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present		2J
TS17: trunk somite	Present	Regionally restricted	2J	Expression in the ventral sites of the somitic buds.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	3A	Ventral expression is high and dorsal expression is low.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Strong		3B
TS17: neural tube lateral wall	Present	Graded	3B	Strong expression in the medial portion and low expression in the dorsal aspect and ventral aspect corresponding to the area of motoneuron differentiation.
TS17: trunk dermomyotome	Weak		3B

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Strong	Regionally restricted	3G	Expression is in the periventricular layers.
TS17: diencephalon	Present	Graded	3G
TS17: telencephalon	Present	Graded	3G
TS17: hindbrain	Present	Graded	3G	Expression is in the metencephalon.

Structure	Level	Pattern	Image	Note
TS20: perioptic mesenchyme	Present		3H

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Regionally restricted	4A	Expression in layers two and three of the cortical plate.
TS26: cerebellum	Present	Regionally restricted	4A	Expression is in the central areas excluding the cerebellar granule cell layer.
TS26: tectum	Present		4A

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	4C	Expression in layers two and three of the cortical plate.

Structure	Level	Pattern	Image	Note
TS26: olfactory lobe	Present	Regionally restricted	4E	Expression is in the mitral cell layer and in the accessory olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	4G	Expression is in the central areas excluding the cerebellar granule cell layer.

Structure	Level	Pattern	Image	Note
TS26: inferior olivary nucleus	Present	Regionally restricted	4I	Expresion is in the medial olivary complex.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	4K	Expression is in the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	4M	Expression is in the fasciola cinereum.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted		Expression is in the habenular nuclei and dorsal thalamic nuclei.
TS26: brain	Present	Regionally restricted		Expression in the rhinencephalon, mammillary nuclei, and in all periventricular layers.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present	Regionally restricted		Expression is in the habenular nuclei and dorsal thalamic nuclei.
TS28: brain	Present	Regionally restricted		Expression in the rhinencephalon, mammillary nuclei, and in all periventricular layers.