Vrk1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83672 Vega FM, et al., Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development. FEBS Lett. 2003 Jun 5;544(1-3):176-80
Assay type:	RT-PCR
MGI Accession ID:	MGI:3027258
Gene symbol:	Vrk1
Gene name:	vaccinia related kinase 1

Probe:	mVRK1A, mVRK1B
Visualized with:	Phosphorimaging
Assay notes:	The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe.

Results

Image: 3A VRK1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	332 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
10.5	E10.5 (a)	TS17: blood	Strong	0.2 µg; total RNA	BALB/c		Not Specified
11.5	E11.5 (a)	TS19: blood	Strong	0.2 µg; total RNA	BALB/c		Not Specified
12.5	E12.5 (a)	TS20: blood	Present	0.2 µg; total RNA	BALB/c		Not Specified
13.5	E13.5 (a)	TS21: blood	Present	0.2 µg; total RNA	BALB/c		Not Specified
14.5	E14.5 (a)	TS22: blood	Present	0.2 µg; total RNA	BALB/c		Not Specified
15.5	E15.5 (a)	TS23: blood	Weak	0.2 µg; total RNA	BALB/c		Not Specified
16.5	E16.5 (a)	TS24: blood	Not Specified	0.2 µg; total RNA	BALB/c		Not Specified
17.5	E17.5 (a)	TS25: blood	Not Specified	0.2 µg; total RNA	BALB/c		Not Specified
18.5	E18.5 (a)	TS26: blood	Not Specified	0.2 µg; total RNA	BALB/c		Not Specified
TKA control	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression level was highest from E10.5-11.5, then slowly decreased until E14.5 and dropped sharply on E15.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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