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Gene Expression Data
Assay Details
Assay
Reference: J:42512 Oliva D, et al., Conserved alternative splicing in the 5'-untranslated region of the muscle-specific enolase gene. Primary structure of mRNAs, expression and influence of secondary structure on the translation efficiency. Eur J Biochem. 1995 Aug 15;232(1):141-9
Assay type: RT-PCR
MGI Accession ID: MGI:2687404
Gene symbol: Eno3
Gene name: enolase 3, beta muscle
Probe: BEN 4, BEN 3
Probe preparation: Double stranded labelled with P32 DNA
Assay notes: The RT-PCR reaction was electrophoresed, Southern blotted and hybridized with a probe (MGI:2687402).
Results Image: 7A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
12d E12.0 (a) TS20: skeletal muscle Present Present 10 µg; total RNA CD-1 Not Specified limb muscle
16d E16.0 (a) TS24: limb musculature Present Present 10 µg; total RNA CD-1 Not Specified
a P adult TS28: limb muscle Present Present 10 µg; total RNA CD-1 Not Specified
12d E12.0 (a) TS20: heart Present Present 10 µg; total RNA CD-1 Not Specified
16d E16.0 (a) TS24: heart Present Present 10 µg; total RNA CD-1 Not Specified
a P adult TS28: heart Present Present 10 µg; total RNA CD-1 Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Ratio of expression of the two alternatively spliced transcripts does not vary during the stages examined.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory