Probe:	Cux2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted		Expression is in the lateral plate mesoderm adjacent to the developing limb buds.
TS15: neural tube	Absent

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	1A	Expression is in the lateral plate mesoderm.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	1A	Expression is in the distal portion.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	1A	Expression is in the distal portion.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1A	Expression is in the progress zone.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	1E	Expression is in the lateral plate mesoderm.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	1E	Expression is in the distal portion.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	1E	Expression is in the distal portion.
TS17: olfactory epithelium	Weak	Regionally restricted	1E	Expression is in the distal olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	1I	Expression is in the lateral plate mesoderm.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	1I	Expression is in the distal portion.
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	1I	Expression is in the distal portion.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	1I	Expression is in the distal portion.
TS17: 1st branchial arch mandibular component ectoderm	Absent		1I

Structure	Level	Pattern	Image	Note
TS19: handplate mesenchyme	Present	Regionally restricted	1M	Expression is in the progress zone.
TS19: handplate apical ectodermal ridge	Absent		1M

Structure	Level	Pattern	Image	Note
TS19: maxillary process	Weak	Regionally restricted		Expression is in the proximal component undergoing outgrowth from the face.
TS19: mandible primordium	Absent

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Regionally restricted	2A	Expression is in the future ossifying region of the cranial vault.
TS20: nasal cavity epithelium	Present		2A
TS20: oral epithelium	Present		2A
TS20: forelimb digit	Present		2A

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Weak
TS20: spinal cord roof plate	Present
TS20: spinal cord mantle layer	Present	Regionally restricted		Expression is in the dorsal sensory neurons and the developing motor nuclei.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Present		2B

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present		2C

Structure	Level	Pattern	Image	Note
TS17: genitourinary system	Present	Regionally restricted	3A	Expression is in a pair of genital swellings.
TS17: cloacal membrane	Absent		3A

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present	Regionally restricted	3B	Expression is in the dorsal and ventral surfaces of the genital tubercle.
TS19: urinary system	Absent		3B	Expression is absent in the urethral plate.

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present		3C
TS20: ureter	Absent		3C	Absent in the urethral epithelium.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present		3D	Expression is in the preputial swellings emerging on the lateral genital tubercle.
TS21: ureter	Absent		3D	Absent in the urethral epithelium.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4A	Expression is in the developing motor neurons. There is overlap in expresion with Isl1.
TS17: neural tube roof plate	Present		4A
TS17: dorsal root ganglion	Present		4A	There is overlap in expresion with Isl1.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4B	Expression is in the developing motor neurons. There is some co-expression with Lim1 in the ventral-most domain.
TS17: neural tube roof plate	Present		4B

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4C	Expression is in the developing motor neurons.
TS17: neural tube roof plate	Present		4C

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		4D	There is overlap in expresion with Isl1.
TS19: dorsal root ganglion	Present		4D	There is overlap in expresion with Isl1.

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		4E	There is some co-expression with Lim1 in the ventral-most domain.

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Present		4F

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Present		4I
TS19: neural tube roof plate	Present		4I
TS19: dorsal root ganglion	Weak		4I