Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33825
Brown-Borg HM, et al., Developmental aspects of prolactin receptor gene expression in fetal and neonatal mice. Eur J Endocrinol. 1996 Jun;134(6):751-7
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2686993
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Gene symbol:
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Prlr
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Gene name:
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prolactin receptor
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Probe:
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PR1, PR2
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Visualized with:
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Ethidium bromide
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Assay notes:
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The RT-PCR reaction was electrophoresed, Southern blotted, hybridized with an oligonucleotide probe (MGI:2687017) labeled with digoxigenin and visualized with chemiluminescence. The result can be seen in the lower blot of Figure 2. No obvious differences in expression were detected between the sexes at E18.5, 19.5, and P1 (other ages not specified).
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Results
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Image: 2_upper blot
| E12.5
(a)
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TS20: liver
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| E13.5
(a)
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TS21: liver
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| E14.5
(a)
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TS22: liver
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| E18.5
(a)
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TS26: liver
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| P1
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TS27: liver
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| E12.5
(a)
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TS20: brain
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
Author also referred to this tissue as cranium tissues.
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| E12.5
(a)
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TS20: brain
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
Author also referred to this tissue as cranium tissues.
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| E13.5
(a)
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TS21: brain
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
Author also referred to this tissue as cranium tissues.
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| E13.5
(a)
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TS21: brain
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
Author also referred to this tissue as cranium tissues.
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| E14.5
(a)
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TS22: head
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
Author also referred to this tissue as brain and cranium tissues.
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| E18.5
(a)
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TS26: brain
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| P1
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TS27: brain
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| E15.5
(a)
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TS23: metanephros
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Pooled |
n=3 samples pooled.
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| E18.5
(a)
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TS26: metanephros
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| P1
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TS27: metanephros
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
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| Mol. Wt. Marker Lane |
Notes:
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