Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33825
Brown-Borg HM, et al., Developmental aspects of prolactin receptor gene expression in fetal and neonatal mice. Eur J Endocrinol. 1996 Jun;134(6):751-7
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2686992
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Gene symbol:
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Prlr
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Gene name:
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prolactin receptor
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Probe:
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PR1, PR2
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Visualized with:
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Ethidium bromide
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Assay notes:
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The RT-PCR reaction was electrophoresed, Southern blotted, hybridized with an oligonucleotide probe (MGI:2687017) labeled with digoxigenin and visualized with chemiluminescence. The result can be seen in the lower blot of Figure 1.
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Results
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Image: 1_upper blot
| E10.5
(a)
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TS17: embryo
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| E10.5
(a)
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TS17: embryo
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Absent
|
3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| E10.5
(a)
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TS17: embryo
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Absent
|
3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| E10.5
(a)
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TS17: embryo
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Absent
(b)
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| E10.5
(a)
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TS17: embryo
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: bladder
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: bladder
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: stomach
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Weak
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: stomach
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Weak
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: skin
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| P2
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TS27: skin
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Absent
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| Control |
| E14.5
(a)
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TS22: liver
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Present
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3 µg; total RNA |
involves: C3H * C57BL/6 |
| Not Specified |
| Mol. Wt. Marker Lane |
Notes:
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Analysis Tools
