Pax2 RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:12768 Fickenscher HR, et al., Murine Pax-2 protein is a sequence-specific trans-activator with expression in the genital system. DNA Cell Biol. 1993 Jun;12(5):381-91
Assay type:	RNase protection
MGI Accession ID:	MGI:2686839
Gene symbol:	Pax2
Gene name:	paired box 2

Probe:	Pax2 cDNA3
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography
Assay notes:	The strain carrying the Ar<Tfm> mutation is also segregating the Eda<Ta> mutation.

Results

Image: 7

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (a)	505 bp	390 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
NMRI	P w 4	TS28: testis	Absent	Absent	Absent	Absent	40 µg; total RNA	NMRI		Male
Tfm	P w 4	TS28: testis	Present	Present	Absent	Absent	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Y	Male
Tfm	P w 6	TS28: testis	Present	Present	Absent	Absent	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Y	Male
Tfm	P w 9	TS28: testis	Present	Present	Absent	Absent	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Y	Male
Tfm	P w 12	TS28: testis	Present	Present	Absent	Absent	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Y	Male
Ta	P w 4	TS28: testis	Absent	Absent	Absent	Absent	40 µg; total RNA	involves: B6CBACa A^w-J/A-Eda^Ta/O	Eda^Ta/Y	Male
Tfm	P w 4	TS28: reproductive system	Absent	Absent	Not Specified	Not Specified	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified	genital region
Tfm	P w 9	TS28: reproductive system	Absent	Absent	Not Specified	Not Specified	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified	genital region
Tfm	P w 12	TS28: reproductive system	Absent	Absent	Not Specified	Not Specified	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified	genital region
NMRI	P w 4	TS28: epididymis	Absent	Absent	Present	Present	40 µg; total RNA	NMRI		Male
Ta	P w 4	TS28: epididymis	Absent	Absent	Not Specified	Not Specified	40 µg; total RNA	involves: B6CBACa A^w-J/A-Eda^Ta/O	Eda^Ta/Y	Male
NMRI	P w 4	TS28: brain	Absent	Absent	Present	Present	40 µg; total RNA	NMRI		Not Specified
Tfm	P w 4	TS28: brain	Absent	Absent	Present	Present	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified
Tfm	P w 6	TS28: brain	Absent	Absent	Present	Present	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified
Tfm	P w 9	TS28: brain	Absent	Absent	Present	Present	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified
Tfm	P w 12	TS28: brain	Absent	Absent	Present	Present	40 µg; total RNA	involves: C57BL/6J-A^w-J * CBA	Ar^Tfm/Ar^Tfm	Not Specified
Ta	P w 4	TS28: brain	Absent	Absent	Not Specified	Not Specified	40 µg; total RNA	involves: B6CBACa A^w-J/A-Eda^Ta/O	Eda^Ta/Eda^Ta	Not Specified
NMRI	P w 4	TS28: metanephros	Absent	Absent	Present	Present	40 µg; total RNA	NMRI		Not Specified
marker	Mol. Wt. Marker Lane
probe	Control

Notes:
(a) This band was only observed in mutant testis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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