Ntrk3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:41123 Wyatt S, et al., Sympathetic neuron survival and TrkA expression in NT3-deficient mouse embryos. EMBO J. 1997 Jun 2;16(11):3115-23
Assay type:	RT-PCR
MGI Accession ID:	MGI:2686672
Gene symbol:	Ntrk3
Gene name:	neurotrophic tyrosine kinase, receptor, type 3

Probe:	trkC-pC, trkC-pD
Assay notes:	Shown are levels of transcripts relative to GAPDH. Primer set amplifies all trkC transcripts. Levels of transcripts lacking the tyrosine kinase domain (TK-) were calculated by subtracting TK+ levels (assayed using primers trkC-pA and trkC-pB; see assayMGI:2686671) from total trkC levels.

Results

Image: 5_TrkC TK-

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E13	E13.0 (a)	TS21: superior cervical ganglion	Present (c)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified
E14	E14.0 (a)	TS22: superior cervical ganglion	Present (c)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified
E15	E15.0 (a)	TS23: superior cervical ganglion	Present (c)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified
E16	E16.0 (a)	TS24: superior cervical ganglion	Present (d)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified
E18	E18.0 (a)	TS26: superior cervical ganglion	Present (d)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified
P1	P1	TS27: superior cervical ganglion	Present (d)	1 µg; poly-A+ RNA	involves: 129S4/SvJae * BALB/c		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference. Authors state the precise stage of development was determined by the criteria of Theiler (1972).
(b) TK- levels were substantially higher than TK+ levels.
(c) Highest expression between E13 and E15; 30- to 40-fold higher than that of TK+.
(d) Levels decreased with age.

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